Back to Search
Start Over
SARS-CoV-2 elicits robust adaptive immune responses regardless of disease severity
- Source :
- Nielsen, S S F, Vibholm, L K, Johannsen, I M, Olesen, R, Frattari, G, Pahus, M H, Højen, J F, Damsgaard Gunst, J, Erikstrup, C, Holleufer, A, Hartmann, R, Østergaard, L J, Søgaard, O S, Schleimann, M H & Tolstrup, M 2021, ' SARS-CoV-2 elicits robust adaptive immune responses regardless of disease severity ', EBioMedicine, vol. 68, 103410 . https://doi.org/10.1016/j.ebiom.2021.103410, EBioMedicine, EBioMedicine, Vol 68, Iss, Pp 103410-(2021)
- Publication Year :
- 2021
-
Abstract
- Background: The SARS-CoV-2 pandemic currently prevails worldwide. To understand the immunological signature of SARS-CoV-2 infections and aid the search and evaluation of new treatment modalities and vaccines, comprehensive characterization of adaptive immune responses towards SARS-CoV-2 is needed.Methods: We included 203 recovered SARS-CoV-2 infected patients in Denmark between April 3rd and July 9th 2020, at least 14 days after COVID-19 symptom recovery. The participants had experienced a range of disease severities from asymptomatic to severe. We collected plasma, serum and PBMC's for analysis of SARS-CoV-2 specific antibody response by Meso Scale analysis including other coronavirus strains, ACE2 competition, IgA ELISA, pseudovirus neutralization capacity, and dextramer flow cytometry analysis of CD8+ T cells. The immunological outcomes were compared amongst severity groups within the cohort, and 10 pre-pandemic SARS-CoV-2 negative controls.Findings: We report broad serological profiles within the cohort, detecting antibody binding to other human coronaviruses. 202(>99%) participants had SARS-CoV-2 specific antibodies, with SARS-CoV-2 neutralization and spike-ACE2 receptor interaction blocking observed in 193(95%) individuals. A significant positive correlation (r=0.7804) between spike-ACE2 blocking antibody titers and neutralization potency was observed. Further, SARS-CoV-2 specific CD8+ T-cell responses were clear and quantifiable in 95 of 106(90%) HLA-A2+ individuals.Interpretation: The viral surface spike protein was identified as the dominant target for both neutralizing antibodies and CD8+ T-cell responses. Overall, the majority of patients had robust adaptive immune responses, regardless of their disease severity.Funding: This study was supported by the Danish Ministry for Research and Education (grant# 0238-00001B) and The Danish Innovation Fund (grant# 0208-00018B)
- Subjects :
- Male
0301 basic medicine
Medicine (General)
Denmark
Adaptive Immunity
CD8-Positive T-Lymphocytes
Antibodies, Viral
medicine.disease_cause
Severity of Illness Index
Neutralization
Serology
0302 clinical medicine
Medicine
Coronavirus
biology
CD8 T-cell
General Medicine
Middle Aged
Acquired immune system
Adaptive
Asymptomatic
Virus
030220 oncology & carcinogenesis
Spike Glycoprotein, Coronavirus
Female
Angiotensin-Converting Enzyme 2
Antibody
Research Paper
Adult
General Biochemistry, Genetics and Molecular Biology
Cell Line
Young Adult
03 medical and health sciences
R5-920
Immune system
Blocking antibody
Animals
Humans
Immune response
Aged
Severe
business.industry
SARS-CoV-2
COVID-19
Antibodies, Neutralizing
030104 developmental biology
Immunology
biology.protein
Corona
CD8+ T-cell
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Nielsen, S S F, Vibholm, L K, Johannsen, I M, Olesen, R, Frattari, G, Pahus, M H, Højen, J F, Damsgaard Gunst, J, Erikstrup, C, Holleufer, A, Hartmann, R, Østergaard, L J, Søgaard, O S, Schleimann, M H & Tolstrup, M 2021, ' SARS-CoV-2 elicits robust adaptive immune responses regardless of disease severity ', EBioMedicine, vol. 68, 103410 . https://doi.org/10.1016/j.ebiom.2021.103410, EBioMedicine, EBioMedicine, Vol 68, Iss, Pp 103410-(2021)
- Accession number :
- edsair.doi.dedup.....39b98eaf4d593544cae2c6e1c0759fba