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Mechanistic insights into how CMG helicase facilitates replication past DNA roadblocks
- Source :
- DNA Repair. 55:76-82
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Before leaving the house, it is a good idea to check for road closures that may affect the morning commute. Otherwise, one may encounter significant delays arriving at the destination. While this is commonly true, motorists may be able to consult a live interactive traffic map and pick an alternate route or detour to avoid being late. However, this is not the case if one needs to catch the train which follows a single track to the terminus; if something blocks the track, there is a delay. Such is the case for the DNA replisome responsible for copying the genetic information that provides the recipe of life. When the replication machinery encounters a DNA roadblock, the outcome can be devastating if the obstacle is not overcome in an efficient manner. Fortunately, the cell's DNA synthesis apparatus can bypass certain DNA obstructions, but the mechanism(s) are still poorly understood. Very recently, two papers from the O'Donnell lab, one structural (Georgescu et al., 2017 [1]) and the other biochemical (Langston and O'Donnell, 2017 [2]), have challenged the conventional thinking of how the replicative CMG helicase is arranged on DNA, unwinds double-stranded DNA, and handles barricades in its path. These new findings raise important questions in the search for mechanistic insights into how DNA is copied, particularly when the replication machinery encounters a roadblock.
- Subjects :
- DNA Replication
0301 basic medicine
DNA damage
DNA replisome
Computer security
computer.software_genre
Biochemistry
DNA Adducts
03 medical and health sciences
chemistry.chemical_compound
Animals
Humans
Molecular Biology
Genetics
Copying
Bacteria
biology
DNA synthesis
DNA Helicases
DNA replication
Eukaryota
Helicase
DNA
Cell Biology
Replication (computing)
030104 developmental biology
chemistry
biology.protein
computer
Subjects
Details
- ISSN :
- 15687864
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- DNA Repair
- Accession number :
- edsair.doi.dedup.....39c3aeb418edbc11a5992721c9ff8532