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Cloning and Expression Study of the Mouse Tetrodotoxin-Resistant Voltage-Gated Sodium Channel α Subunit NaT/Scn11a
- Source :
- Biochemical and Biophysical Research Communications. 267:271-277
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- We have cloned a tetrodotoxin-resistant (TTX-R) voltage-gated sodium channel α subunit from a mouse cDNA library and designated it as NaT. It encodes 1765 amino acid residues and is virtually identical to that of Scn11a, which has been reported recently, except for 40 nt and 14 aa substitutions. The amino acid identity of NaT/Scn11a with rat NaN/SNS2 is 88%. NaT/Scn11a was mapped to mouse chromosome 9F3-F4 by fluorescence in situ hybridization (FISH). While rat NaN/SNS2 has been reported to be expressed specifically in the peripheral sensory neurons, NaT/Scn11a is expressed not only in the peripheral sensory neurons but also in the spinal cord, uterus, testis, ovary, placenta, and small intestine. NaT is detectable in mouse embryos 15 days postcoitus (p.c.), around the phase of organogenesis and gonadal differentiation. These findings demonstrate a unique distribution of NaT/Scn11a and suggest some of its roles in the above-mentioned processes.
- Subjects :
- Male
endocrine system
Molecular Sequence Data
Biophysics
Tetrodotoxin
Biology
Biochemistry
Sodium Channels
Embryonic and Fetal Development
Mice
Pregnancy
Placenta
medicine
Animals
Amino Acid Sequence
Neurons, Afferent
Peripheral Nerves
Cloning, Molecular
Molecular Biology
In Situ Hybridization, Fluorescence
Gene Library
chemistry.chemical_classification
Cloning
Sequence Homology, Amino Acid
medicine.diagnostic_test
Reverse Transcriptase Polymerase Chain Reaction
cDNA library
Sodium channel
fungi
Chromosome Mapping
Gene Expression Regulation, Developmental
Embryo
Cell Biology
Molecular biology
Recombinant Proteins
Rats
Amino acid
body regions
medicine.anatomical_structure
chemistry
Organ Specificity
Nat
Karyotyping
Female
Sequence Alignment
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 267
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....3a05e2c52376378a1862c388bb041e96