Back to Search
Start Over
Over Expression of NANOS3 and DAZL in Human Embryonic Stem Cells
- Source :
- PLoS ONE, Vol 11, Iss 10, p e0165268 (2016), PLoS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science, 2016.
-
Abstract
- The mechanisms underlying human germ cell development are largely unknown, partly due to the scarcity of primordial germ cells and the inaccessibility of the human germline to genetic analysis. Human embryonic stem cells can differentiate to germ cells in vitro and can be genetically modified to study the genetic requirements for germ cell development. Here, we studied NANOS3 and DAZL, which have critical roles in germ cell development in several species, via their over expression in human embryonic stem cells using global transcriptional analysis, in vitro germ cell differentiation, and in vivo germ cell formation assay by xenotransplantation. We found that NANOS3 over expression prolonged pluripotency and delayed differentiation. In addition, we observed a possible connection of NANOS3 with inhibition of apoptosis. For DAZL, our results suggest a post-transcriptional regulation mechanism in hES cells. In addition, we found that DAZL suppressed the translation of OCT4, and affected the transcription of several genes associated with germ cells, cell cycle arrest, and cell migration. Furthermore, DAZL over expressed cells formed spermatogonia-like colonies in a rare instance upon xenotransplantation. These data can be used to further elucidate the role of NANOS3 and DAZL in germ cell development both in vitro and in vivo.
- Subjects :
- 0301 basic medicine
Male
Transcription, Genetic
Cellular differentiation
Protein Expression
Gene Expression
lcsh:Medicine
Biochemistry
Germline
DAZL
Animal Cells
Medicine and Health Sciences
Testes
lcsh:Science
Staining
Multidisciplinary
Messenger RNA
RNA-Binding Proteins
Cell Staining
Cell migration
Cell Differentiation
Transfection
Cell biology
Nucleic acids
medicine.anatomical_structure
Heterografts
Female
Germ line development
Cellular Types
Anatomy
Genital Anatomy
Germ cell
Research Article
endocrine system
Biology
Research and Analysis Methods
03 medical and health sciences
medicine
Genetics
Gene Expression and Vector Techniques
Humans
RNA, Messenger
Molecular Biology Techniques
Molecular Biology
Embryonic Stem Cells
Molecular Biology Assays and Analysis Techniques
030102 biochemistry & molecular biology
lcsh:R
Reproductive System
Biology and Life Sciences
Cell Biology
Molecular biology
Embryonic stem cell
030104 developmental biology
Germ Cells
Specimen Preparation and Treatment
RNA
lcsh:Q
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....3a45b103a20ac4412ee4738d05364933