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Genomic characterization of human rotavirus G10 strains from the African Rotavirus Network: relationship to animal rotaviruses

Authors :
Krisztián Bányai
Jon R. Gentsch
J.C. Forbi
Kimberly Foytich
Jennifer J. Hull
Mathew D. Esona
Tara Kerin
V.A. Agbaya
Nicola Page
Michael D. Bowen
James Nyangao
George Armah
A. Geyer
Roger I. Glass
Slavica Mijatovic-Rustempasic
Molly M. Freeman
A. D. Steele
M. Aminu
Rashi Gautam
L.M. Seheri
Source :
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 11(1)
Publication Year :
2010

Abstract

Global rotavirus surveillance has led to the detection of many unusual human rotavirus (HRV) genotypes. The aim of this study was to elucidate the genetic and evolutionary relationships of short fragments of all 11 gene segments of G10 HRV strains identified in West Africa through the African Rotavirus Network (ARN) system. During 1998–2004 surveillance within the ARN, we identified 5 G10 P[8] HRV strains. Fragments of all 11 gene segments of these G10 strains were sequenced. Phylogenetic and sequence analyses of each gene segment revealed high nucleotide similarities amongst the ARN strains (97–100%) except in the case of the VP1(85–96%) and NSP2 genes (87.8–99.7%) where some strains were divergent. All genes of the ARN strains were classified as Wa-like (genotype 1) with the exception of their VP7 gene of all strains (genotype G10) and the VP6 gene of a single strain, 6755/2002/ARN (DS-1 like, genotype 2). While classified as Wa-like, the NSP2 genes of four of the ARN strains occupied a distinct sub-lineage related to simian strain Tuch, while the NSP2 of strain 6755/2002/ARN and NSP5 genes of all strains were closely related to the cognate genes of both human and animal strains belonging to the Wa-like genogroup. Although these findings help to elucidate the evolution of ARN G10 strains, additional sequence studies of cognate animal rotavirus genes are needed to determine irrefutably the specific origin of those genes relative to both human and animal rotavirus strains.

Details

ISSN :
15677257
Volume :
11
Issue :
1
Database :
OpenAIRE
Journal :
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
Accession number :
edsair.doi.dedup.....3a530e94731f08e903a86d20b9e7faab