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ZNF148 modulates TOP2A expression and cell proliferation via ceRNA regulatory mechanism in colorectal cancer
- Source :
- Medicine
- Publication Year :
- 2017
-
Abstract
- Background: Competing endogenous RNA (ceRNA) regulation is a novel hypothesized mechanism that states RNA molecules share common target microRNAs (miRNAs) and may competitively combine into the same miRNA pool. Methods: Zinc finger protein 148 (ZNF148) and TOP2A expression were analyzed in 742 colorectal cancer (CRC) tissues using immunohistochemistry (IHC). ZNF148 mRNA, TOP2A mRNA, miR101, miR144, miR335, and miR365 expression were estimated in 53 fresh frozen CRC tissues by reverse transcription polymerase chain reaction. Mechanisms underpinning ceRNA were examined using bioinformatics, correlation analysis, RNA interference, gene over-expression, and luciferase assays. Results: Protein levels of ZNF148 and TOP2A detected by IHC positively correlated (Spearman correlation coefficient [rs] = 0.431, P
- Subjects :
- 0301 basic medicine
03 medical and health sciences
0302 clinical medicine
RNA interference
Antigens, Neoplasm
microRNA
Medicine
Humans
competing endogenous RNA
Poly-ADP-Ribose Binding Proteins
Cell Proliferation
Regulation of gene expression
Genetics
Gene knockdown
Messenger RNA
Competing endogenous RNA
business.industry
Gene Expression Profiling
RNA
Zinc Fingers
TOP2A
General Medicine
Clinical Trial/Experimental Study
colorectal neoplasms
Molecular biology
Immunohistochemistry
Reverse transcription polymerase chain reaction
DNA-Binding Proteins
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
DNA Topoisomerases, Type II
030220 oncology & carcinogenesis
ZNF148
business
Transcription Factors
Research Article
Subjects
Details
- ISSN :
- 15365964
- Volume :
- 96
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Medicine
- Accession number :
- edsair.doi.dedup.....3a5fc11bed36cf6d1637a262e77f79d4