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The Additional Detrimental Effects of Cold Preservation on Transplantation-Associated Injury in Kidneys from Living and Brain-Dead Donor Rats
- Source :
- Transplantation, 87(1), 52-58. LIPPINCOTT WILLIAMS & WILKINS
- Publication Year :
- 2009
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2009.
-
Abstract
- Background. Brain death and cold preservation are major alloantigen-independent risk factors for transplantation Outcome. The present study was conducted to assess the influence of these factors on transplantation-associated injury independently or in combination.Methods. Brain death was induced in F344 rats. Renal grafts were harvested after 6 hr and either directly transplanted in unilateral nephrectomized Lewis recipient or Subjected to 24 hr of cold preservation in University of Wisconsin solution before implantation. Allografts obtained from living donor rats were also subjected to cold preservation or not. DNA damage was assessed before implantation by terminal deoxynucleotide transferase-mediated dUTP nick-end labeling staining. Ten days after transplantation, renal histology was performed according to Banff '97 classification. The expressions of cytokines and adhesion molecules were analyzed by quantitative polymerase chain reaction.Results. Cold preservation significantly increased the number of terminal deoxynucleotide transferase-mediated dUTP nick-end labeling positive cells in renal allografts. Ten days after transplantation, histology revealed a higher degree of tubulitis and vasculitis scores when the grafts were Subjected to cold storage. Vasculitis was aggravated when the graft was obtained from brain death (BD) donors. BD, but not cold preservation alone, was associated with papillary necrosis. This was more frequently observed after cold preservation. Immunohistology showed an increase in MHC class II+ cells after cold preservation. The combination of BD and cold preservation revealed a higher degree of VEGF and IL-10 expression.Conclusions. Our Study emphasizes that cold ischemia time should be limited when renal allografts from brain-dead donors are transplanted.
- Subjects :
- Male
Brain Death
Pathology
medicine.medical_specialty
Cold storage
Cold preservation
Biology
Kidney
Cold Ischemia Time
PAPILLARY NECROSIS
ACTIVATION
SYSTEMIC INTERLEUKIN-10 RELEASE
HYPOTHERMIC PRESERVATION
Ischemia
medicine
DOPAMINE TREATMENT
Animals
Transplantation, Homologous
Viaspan
ALLOGRAFT-REJECTION
GRAFT-SURVIVAL
Cryopreservation
Transplantation
Renal inflammation
RENAL-TRANSPLANTATION
Histocompatibility Antigens Class II
Renal transplantation
Histology
DNA
Organ Preservation
medicine.disease
Kidney Transplantation
PROXIMAL TUBULAR CELLS
Rats
Staining
Cold Temperature
surgical procedures, operative
Real-time polymerase chain reaction
ENDOTHELIAL GROWTH-FACTOR
Cytokines
Vasculitis
DNA Damage
Subjects
Details
- ISSN :
- 00411337
- Volume :
- 87
- Database :
- OpenAIRE
- Journal :
- Transplantation
- Accession number :
- edsair.doi.dedup.....3a72c9a0493dac170749e3745b67a677
- Full Text :
- https://doi.org/10.1097/tp.0b013e318191b2ca