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Human CD200 suppresses macrophage-mediated xenogeneic cytotoxicity and phagocytosis
- Source :
- Surgery Today. 48:119-126
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Various strategies, such as the generation of alpha-1,3-galactosyltransferase knocked-out pigs and CD55 transgenic pigs, have been investigated to inhibit pig to human xenogeneic rejection. Our aim is to develop strategies to overcome the hurdle of not only hyper acute rejection, but also that of cellular xenogeneic rejection (CXR). Although macrophages have been well known to play a critical role in CXR, monocyte/macrophage-mediated xenogeneic rejection has not been well studied. In this study, we evaluated the effect of CD200 in xenogeneic rejection by macrophages. Naive swine endothelial cells (SEC) and SEC/CD200 were co-cultured with M0 macrophages and the cytotoxicity was measured by a WST-8 assay. The phagocytosis of SEC and SEC/CD200 by macrophages was analyzed by flow cytometry. While CD200 failed to suppress a significant amount of cytotoxicity against SEC by monocytes, M0 macrophage-mediated cytotoxicity was significantly suppressed by human CD200. The phagocytosis by M0 macrophages was also tested. The phagocytosis assay revealed that human CD200 suppresses M0 macrophage-mediated phagocytosis. Our findings indicate that human CD200 suppresses the xenogeneic rejection by CD200R+ macrophages and that the generation of hCD200 transgenic pigs for use in xenografts is very attractive for preventing the macrophage-mediated rejection.
- Subjects :
- Cytotoxicity, Immunologic
Graft Rejection
0301 basic medicine
Swine
Phagocytosis
Transgene
Xenotransplantation
medicine.medical_treatment
Biology
Flow cytometry
03 medical and health sciences
0302 clinical medicine
Antigens, CD
medicine
Animals
Humans
Macrophage
Cytotoxicity
Cells, Cultured
medicine.diagnostic_test
Macrophages
Monocyte
Endothelial Cells
General Medicine
Flow Cytometry
Molecular biology
030104 developmental biology
medicine.anatomical_structure
Phagocytosis assay
Surgery
030215 immunology
Subjects
Details
- ISSN :
- 14362813 and 09411291
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Surgery Today
- Accession number :
- edsair.doi.dedup.....3accce21e76dc840bddfc4cecf24a54c