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In vitro study in the endothelial cell compatibility and endothelialization of genipin-crosslinked biological tissues for tissue-engineered vascular scaffolds

Authors :
Xu Yuan-ting
Liu Fei
Wan Chang-xiu
Yu Xi-xun
Source :
Journal of Materials Science: Materials in Medicine. 21:777-785
Publication Year :
2009
Publisher :
Springer Science and Business Media LLC, 2009.

Abstract

To overcome the cytotoxicity of the chemical reagents used to fix bioprostheses, genipin, a naturally occurring crosslinking agent, was used to fix biological tissues in present study. We prepared the biological vascular scaffolds through cell extraction and fixing the porcine thoracic arteries with 1% (by w/v) genipin solution for 3 days, and then examined their mechanical properties and microstructures; glutaraldehyde- and epoxy-fixed counterparts were used as controls. HUVECs were seeded on the type I collagen-coated surface of different modified acellular vascular tissues (fixed with different crosslinking agents), and the growths of HUVECs on the specimens were demonstrated by means of MTT test, the secretion of PGI2 and vWF by HUVECs on the various specimens was also measured. Finally, HUVECs were seeded on the luminal surface of acellular biological vascular scaffolds (

Details

ISSN :
15734838 and 09574530
Volume :
21
Database :
OpenAIRE
Journal :
Journal of Materials Science: Materials in Medicine
Accession number :
edsair.doi.dedup.....3b0896c579f045809e2474a95916b529