Back to Search
Start Over
Putting the Hindgut Hypothesis to the Test in a Diabetic Zucker Rat Model
- Source :
- Obesity Surgery. 29:4000-4007
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- The hindgut theory hypothesizes a key role of differential hindgut stimulation following metabolic procedures in ameliorating diabetes mellitus. We used two strategies to remove the hindgut from intestinal continuity in order to analyze its impact on diabetes mellitus. Loop duodeno-jejunostomy (DJOS) with exclusion of one-third of total intestinal length was performed in 3 groups of 9-week-old Zucker diabetic fatty rats. In group 1, no further alteration of the intestinal tract was made. Group 2 received additional ileal exclusion (IE). Group 3 underwent additional resection of 50% of the ileum with side-to-side ileocecal anastomosis (IR). One, 2, and 4 months after surgery, fasting blood glucose levels, oral glucose tolerance tests (OGTT), and glucose-stimulated hormone analyses were conducted, and bile acid blood levels were compared. Body weight was documented weekly. In relation to DJOS, glucose control was not impaired in IR or IE. On the contrary, only IR could maintain preOP glucose values until 4 months. There were no significant weight differences between the groups. Confirming effective ileal diversion, bile acid blood levels were significantly higher in the DJOS group compared with both IR and IE (p = 0.0025 and p = 0.0047). Operative interventions had no impact on GLP-1 levels at any time point (ANOVA p > 0.05 for all). Insulin secretion was preserved in all groups. This data supports the hypothesis that the mechanisms driving amelioration of diabetes mellitus are complex and cannot be reduced to the ileum.
- Subjects :
- Blood Glucose
Male
medicine.medical_specialty
Duodenum
medicine.drug_class
Endocrinology, Diabetes and Metabolism
Rat model
030209 endocrinology & metabolism
Ileum
Stimulation
Diabetes Mellitus, Experimental
Bile Acids and Salts
Random Allocation
03 medical and health sciences
0302 clinical medicine
Glucagon-Like Peptide 1
Internal medicine
Diabetes mellitus
Glucose Intolerance
Intestine, Small
medicine
Animals
Insulin
Nutrition and Dietetics
Bile acid
business.industry
Hindgut
Glucose Tolerance Test
medicine.disease
Rats
Rats, Zucker
Jejunum
medicine.anatomical_structure
Endocrinology
030211 gastroenterology & hepatology
Surgery
Analysis of variance
business
Biomarkers
Hormone
Subjects
Details
- ISSN :
- 17080428 and 09608923
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Obesity Surgery
- Accession number :
- edsair.doi.dedup.....3b14d0c75a4c7ee2522927ec023602f9