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Redistribution of Extracellular Superoxide Dismutase Causes Neonatal Pulmonary Vascular Remodeling and PH but Protects Against Experimental Bronchopulmonary Dysplasia
- Source :
- Antioxidants; Volume 7; Issue 3; Pages: 42, Antioxidants, Antioxidants, Vol 7, Iss 3, p 42 (2018)
- Publication Year :
- 2018
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2018.
-
Abstract
- Background: A naturally occurring single nucleotide polymorphism (SNP), (R213G), in extracellular superoxide dismutase (SOD3), decreases SOD3 matrix binding affinity. Humans and mature mice expressing the R213G SNP exhibit increased cardiovascular disease but decreased lung disease. The impact of this SNP on the neonatal lung at baseline or with injury is unknown. Methods: Wild type and homozygous R213G mice were injected with intraperitoneal bleomycin or phosphate buffered saline (PBS) three times weekly for three weeks and tissue harvested at 22 days of life. Vascular and alveolar development were evaluated by morphometric analysis and immunostaining of lung sections. Pulmonary hypertension (PH) was assessed by right ventricular hypertrophy (RVH). Lung protein expression for superoxide dismutase (SOD) isoforms, catalase, vascular endothelial growth factor receptor 2 (VEGFR2), endothelial nitric oxide synthase (eNOS) and guanosine triphosphate cyclohydrolase-1 (GTPCH-1) was evaluated by western blot. SOD activity and SOD3 expression were measured in serum. Results: In R213G mice, SOD3 lung protein expression decreased, serum SOD3 protein expression and SOD serum activity increased compared to wild type (WT) mice. Under control conditions, R213G mice developed pulmonary vascular remodeling (decreased vessel density and increased medial wall thickness) and PH; alveolar development was similar between strains. After bleomycin injury, in contrast to WT, R213G mice were protected from impaired alveolar development and their vascular abnormalities and PH did not worsen. Bleomycin decreased VEGFR2 and GTPCH-1 only in WT mice. Conclusion: R213G neonatal mice demonstrate impaired vascular development and PH at baseline without alveolar simplification, yet are protected from bleomycin induced lung injury and worsening of pulmonary vascular remodeling and PH. These results show that vessel bound SOD3 is essential in normal pulmonary vascular development, and increased serum SOD3 expression and SOD activity prevent lung injury in experimental bronchopulmonary dysplasia (BPD) and PH.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Physiology
SOD3
extracellular superoxide dismutase
Clinical Biochemistry
R213G single nucleotide polymorphism
neonate
bronchopulmonary dysplasia
pulmonary hypertension
Lung injury
Bleomycin
Biochemistry
Article
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
Right ventricular hypertrophy
Internal medicine
medicine
Molecular Biology
Lung
biology
Chemistry
lcsh:RM1-950
Cell Biology
respiratory system
medicine.disease
Pulmonary hypertension
3. Good health
030104 developmental biology
Endocrinology
medicine.anatomical_structure
lcsh:Therapeutics. Pharmacology
Bronchopulmonary dysplasia
biology.protein
Subjects
Details
- Language :
- English
- ISSN :
- 20763921
- Database :
- OpenAIRE
- Journal :
- Antioxidants; Volume 7; Issue 3; Pages: 42
- Accession number :
- edsair.doi.dedup.....3b19e2fb9d9b1efd54bb38e0e30c4b17
- Full Text :
- https://doi.org/10.3390/antiox7030042