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Identification and functional analysis of grouper (Epinephelus coioides) B-cell linker protein BLNK
- Source :
- Fish & Shellfish Immunology. 81:399-407
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- B-cell linker protein (BLNK) is an adaptor protein that plays a crucial role in the B cell antigen receptor (BCR) signal pathway. To investigate the function of BLNK in teleost fish, we cloned a BLNK ortholog gene from the orange-spotted grouper (Epinephelus coioides). Homology analysis showed that the grouper BLNK (EcBLNK) had a 34%-77% amino acid identity in comparison to other vertebrates and shared the highest amino acid identity with BLNK from the Asian seabass Lates calcarifer. EcBLNK comprises an N-terminal SAM domain and a C-terminal B-cell linker SH2 domain. Ten tyrosine residues were well conserved between teleost fish and mammals. Tissue distribution analysis showed that EcBLNK was expressed mainly in immune organs and expression was at the highest level in head kidney. Co-localization of EcBLNK and EcCD79a was observed in transfected HEK293T cells. Overexpression of EcBLNK did not activate nuclear factor kappa-light-chain-enhancer of activated B cells. The protein level of EcBLNK in grouper head kidney leukocytes was increased by stimulation with lipopolysaccharide. In groupers infected with Cryptocaryon irritans, EcBLNK was regulated in the infected sites and the systemic organ which suggests that EcBLNK was activated in the immune response to parasite infection.
- Subjects :
- Fish Proteins
Lipopolysaccharides
0301 basic medicine
Ciliophora Infections
Aquatic Science
Biology
SH2 domain
Fish Diseases
03 medical and health sciences
Leukocytes
Animals
Humans
Environmental Chemistry
Grouper
Tyrosine
Adaptor Proteins, Signal Transducing
chemistry.chemical_classification
030102 biochemistry & molecular biology
HEK 293 cells
breakpoint cluster region
Signal transducing adaptor protein
General Medicine
Head Kidney
biology.organism_classification
Perciformes
Cell biology
Amino acid
HEK293 Cells
030104 developmental biology
chemistry
B-Cell Linker Protein
Subjects
Details
- ISSN :
- 10504648
- Volume :
- 81
- Database :
- OpenAIRE
- Journal :
- Fish & Shellfish Immunology
- Accession number :
- edsair.doi.dedup.....3b37b3de3d5551b5d3deeba7400e198c