Back to Search
Start Over
Preventive effect of Rumex crispus L. on surgically induced intra-abdominal adhesion model in rats
- Source :
- Daru
- Publication Year :
- 2021
-
Abstract
- BACKGROUND: Rumex crispus L. (Polygonaceae), known as “Labada” in Turkey, was reported to be used for the treatment of gynecological diseases such as postpartum complications and infertility in folk medicine. Earlier studies on R. crispus have shown that leaf, fruit and root extracts have anti-inflammatory and antioxidant activities and are used for the treatment of tumors in the uterus. The hypothesis of this study is that R. crispus may generate potential anti-adhesive activity against complex factors such as inflammation, oxidation and fibrosis. OBJECTIVES: We aimed to investigate the potential anti-adhesive activity of aqueous methanol extracts of leaves, fruits and roots of R. crispus. METHODS: Abdominal adhesion model was performed in 72 female Wistar Albino rats. In the first step of the experiment, the rats were divided into six groups namely, Sham, Control, Reference and Experimental Groups (consisting of three sub-groups in which R. crispus leaf, fruit and root extracts were applied at 100 mg/kg dose). The test samples were administered once to the peritoneal cavity and the rats were sacrificied at the end of the 14th day. Root extract showed prominent activity, therefore this extract was subjected to fractionation to obtain 3 fractions (30–60–100% methanol fractions) by using vacuum-liquid chromatography. In the second stage, animals were divided into 6 groups as Sham, Control, Reference and Experimental Groups (R30, R60, R100 at 100 mg/kg dose). Adhesion scoring, tissue total antioxidant and oxidant levels, histopathological and immunohistochemical (TNF-α, IL-6 and IL-8) analyzes were performed. RESULTS AND CONCLUSION: Adhesion scores, inflammatory cytokines and inflammation cells decreased by the application of R. crispus root extract. The fractions also showed similar anti-inflammatory effects, but R60 was found to be more effective in prevention of intra-abdominal adhesions and uterine fibrosis. R60 fraction, possessing potential bioactivity, was investigated in terms of phenolic composition by HPLC. GRAPHICAL ABSTRACT: [Image: see text]
- Subjects :
- Antioxidant
medicine.drug_class
medicine.medical_treatment
Phytochemicals
Uterus
Tissue Adhesions
Pharmacology
Plant Roots
Anti-inflammatory
Polygonaceae
Proinflammatory cytokine
Rats, Sprague-Dawley
03 medical and health sciences
Peritoneal cavity
Postoperative Complications
0302 clinical medicine
Fibrosis
Abdomen
medicine
Animals
Rumex
030304 developmental biology
Uterine Diseases
0303 health sciences
biology
Plant Extracts
business.industry
Building and Construction
biology.organism_classification
medicine.disease
Plant Leaves
Disease Models, Animal
medicine.anatomical_structure
Fruit
030220 oncology & carcinogenesis
Cytokines
Female
business
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Daru
- Accession number :
- edsair.doi.dedup.....3b3943db313fd76c7a37df710db05cdd