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Isolation and gene expression analysis of a papain-type cysteine protease in thermogenic skunk cabbage (Symplocarpus renifolius)
- Source :
- Bioscience, biotechnology, and biochemistry. 76(10)
- Publication Year :
- 2012
-
Abstract
- Skunk cabbage (Symplocarpus renifolius) spadices contain abundant transcripts for cysteine protease (CP). From thermogenic spadices, we isolated SrCPA, a highly expressed CP gene that encoded a papain-type CP. SrCPA is structurally similar to other plant CPs, including the senescence-associated CPs found in aroids. The expression of SrCPA increased during floral development, and was observed in all floral tissues except for the stamens.
- Subjects :
- DNA, Complementary
Molecular Sequence Data
Biology
Applied Microbiology and Biotechnology
Biochemistry
Analytical Chemistry
chemistry.chemical_compound
Gene Expression Regulation, Plant
Gene expression
Papain
Araceae
Amino Acid Sequence
RNA, Messenger
Cloning, Molecular
Molecular Biology
Gene
Peptide sequence
Regulation of gene expression
Cloning
Base Sequence
fungi
Organic Chemistry
food and beverages
General Medicine
Cysteine protease
chemistry
Thermogenesis
Biotechnology
Subjects
Details
- ISSN :
- 13476947
- Volume :
- 76
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Accession number :
- edsair.doi.dedup.....3baf005cadfb58542b1105be2ef0b466