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The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk.) Pat, and its effects on gastric cancer cells
- Source :
- Fitoterapia. 89:210-217
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Trametenolic acid B (TAB), the bioactive component in the Trametes lactinea (Berk.) Pat, was reported to possess cytotoxic activities and thrombin inhibiting effects. This study was performed to investigate the effects of TAB on H(+)/K(+)-ATPase and gastric cancer. The H(+)/K(+)-ATPase inhibitory activity was determined by gastric parietal cells. Compared to the normal control group, TAB (10, 20, 40 and 80 μg/mL) inhibited the H(+)/K(+)-ATPase activity by 15.97, 16.96, 24.86 and 16.25%, respectively. In the study, 36 Kunming mice were randomly divided into six groups: control, model, TAB-L (TAB, 5 mg/kg/day, i.g.), TAB-M (TAB, 20 mg/kg/day, i.g.), TAB-H (TAB, 40 mg/kg/day, i.g.) and omeprazole (OL, 10 mg/kg/day, i.g.). All mice except the control group were administrated with anhydrous alcohol (5.0 mL/kg, i.g.) for induced gastric-ulcer 1h after the 5th day. At the same time, the control mice were given the same volume of physiological saline. After 4h, TAB was evaluated for H(+)/K(+)-ATPase inhibitory activities of ulcerative gaster, gastric ulcer index and ulcer inhibition. In vitro, the anti-proliferation effect of TAB to gastric cancer cell (HGC-27) in acid environment was detected by MTT, and the apoptosis morphological changes were also observed by Hoechst 33258 dye assay. The results indicated that TAB inhibited moderately H(+)/K(+)-ATPase activity in vitro. Compared to the model group, TAB showed anti-ulcer effects in gastric tissue with the dosages of 20 and 5 mg/kg in vivo. Apart from that, TAB could selectively inhibit gastric cancer cell viability and reduce cell apoptosis against HGC-27 cells at low doses in acid environment.
- Subjects :
- Pathology
medicine.medical_specialty
ATPase
Apoptosis
Mice, Inbred Strains
Anti-gastric cancer
Ulcer index
Gastric Acid
H(+)-K(+)-Exchanging ATPase
Mice
Random Allocation
Parietal Cells, Gastric
In vivo
Stomach Neoplasms
Cell Line, Tumor
Trametenolic acid B
Drug Discovery
medicine
Cytotoxic T cell
Animals
Stomach Ulcer
Omeprazole
H+/K+-ATPase inhibitory activity
Cell Proliferation
Trametes
Pharmacology
biology
business.industry
Plant Extracts
Stomach
Proton Pump Inhibitors
General Medicine
Anti-Ulcer Agents
Molecular biology
Antineoplastic Agents, Phytogenic
In vitro
Triterpenes
Docking simulation
Cancer cell
biology.protein
business
medicine.drug
Phytotherapy
Subjects
Details
- ISSN :
- 0367326X
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Fitoterapia
- Accession number :
- edsair.doi.dedup.....3bbec01c6fb5cae70fc7c26c37ce11cc
- Full Text :
- https://doi.org/10.1016/j.fitote.2013.05.021