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Hepatitis C Virus-Specific Cell-Mediated Immune Responses in Children Born to Mothers Infected with Hepatitis C Virus
- Source :
- The Journal of Pediatrics
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Objective To investigate the association between hepatitis C virus (HCV)-specific cell-mediated immunity (CMI) responses and viral clearance in children born to mothers infected with HCV. Study design A cross-sectional study of children from a mother-infant cohort in Egypt were enrolled to detect CMI responses to recombinant core and nonstructural HCV antigens (nonstructural segments NS3, NS4a/b, and NS5 of the HCV genome) using an interferon-gamma enzyme-linked immunospot assay. Children born to mothers with chronic HCV were enrolled into 3 groups: transiently viremic (n = 5), aviremic (n = 36), and positive control (n = 6), which consisted of 1 child with chronic HCV from this cohort and another 5 children with chronic HCV from a companion study. Children without HCV born to mothers without HCV (n = 27) served as a negative control group. Wilcoxon rank sum test was used to compare the magnitude of CMI responses between groups. Results None of the 6 control children who were positive for HCV responded to any HCV antigen, and 4 (80%) of 5 children with transient viremia responded to at least one HCV antigen, compared with 5 (14%) of 36 and 3 (11%) of 27 children in the aviremic and negative control groups, respectively. Children with transient viremia elicited stronger responses than did negative controls (P = .005), positive controls (P = .011), or children without HCV viremia (P = .012), particularly to nonstructural antigens. Conclusions HCV-specific CMI responses were significantly higher in magnitude and frequency among transiently infected children compared with those persistently infected. This suggests CMI responses may be associated with past viral clearance and can identify children at high risk of infection, who can be targeted for health education, screening, and follow-up.
- Subjects :
- Male
NS3/NS4, Nonstructural segments NS3, NS4a, and NS4b of the HCV genome
Hepatitis C virus
Hepacivirus
Mothers
Viremia
Anti-HCV, Antibodies to hepatitis C virus
medicine.disease_cause
SFC, Spot-forming cell
HCV, Hepatitis C virus
03 medical and health sciences
0302 clinical medicine
Antigen
medicine
Humans
Pediatrics, Perinatology, and Child Health
Prospective Studies
030212 general & internal medicine
Child
Prospective cohort study
030304 developmental biology
Immunity, Cellular
0303 health sciences
NS3
IFN-γ, Interferon-gamma
biology
business.industry
ELISPOT
NS5, Nonstructural segment NS5 of the HCV genome
virus diseases
PBMC, Peripheral blood mononuclear cell
Hepatitis C, Chronic
medicine.disease
biology.organism_classification
Virology
digestive system diseases
3. Good health
Cross-Sectional Studies
Child, Preschool
CMI, Cell-mediated immunity
Pediatrics, Perinatology and Child Health
Cohort
Immunology
ELISPOT, Enzyme-linked immunospot assay
Female
Original Article
business
Subjects
Details
- ISSN :
- 00223476
- Volume :
- 162
- Database :
- OpenAIRE
- Journal :
- The Journal of Pediatrics
- Accession number :
- edsair.doi.dedup.....3bcf8d2681dff627a62561cf916a44b3