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Related carbapenemase-producing Klebsiella isolates detected in both a hospital and associated aquatic environment in Sweden
- Source :
- European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 37(12)
- Publication Year :
- 2018
-
Abstract
- Carbapenem antibiotics are one of the last-resort agents against multidrug-resistant (MDR) bacteria. The occurrence of carbapenemase-producing Enterobacteriaceae (CPE) in wastewater and aquatic environments is an indication of MDR bacteria in the community. This study evaluated CPE in aquatic environments and compared them to the local hospital isolates in Sweden. Phenotypic and genotypic analyses of antibiotic resistance of environmental and clinical CPE were performed. The relatedness of the isolates and possible clonal dissemination was evaluated using phylogenetic and phyloproteomic analysis. Klebsiella oxytoca carrying carbapenemase genes (blaVIM-1, blaIMP-29) were isolated from wastewater and the recipient river, while K. oxytoca (blaVIM-1) and Klebsiella pneumoniae (blaVIM-1, blaOXA-48, blaNDM-1, blaKPC-3) were isolated from patients at the local clinics or hospital. The K. oxytoca classified as sequence type 172 (ST172) isolated from the river was genotypically related to two clinical isolates recovered from patients. The similarity between environmental and clinical isolates suggests the dispersion of blaVIM-1 producing K. oxytoca ST172 from hospital to aquatic environment and the likelihood of its presence in the community. This is the first report of CPE in aquatic environments in Sweden; therefore, surveillance of aquatic and hospital environments for CPE in other urban areas is important to determine the major transfer routes in order to formulate strategies to prevent the spread of MDR bacteria.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
DNA, Bacterial
medicine.medical_specialty
Klebsiella
Genotype
Klebsiella pneumoniae
030106 microbiology
Microbial Sensitivity Tests
Multidrug resistance
Wastewater
Antimicrobial resistance
beta-Lactamases
Microbiology
03 medical and health sciences
Medical microbiology
Antibiotic resistance
Bacterial Proteins
Rivers
Drug Resistance, Multiple, Bacterial
medicine
Humans
Carbapenemase-producing Enterobacteriaceae
Phylogeny
Sweden
Cross Infection
biology
Whole Genome Sequencing
Extended spectrum beta-lactamase
Klebsiella oxytoca
General Medicine
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Enterobacteriaceae
Hospitals
Klebsiella Infections
Multiple drug resistance
030104 developmental biology
Infectious Diseases
Carbapenem-Resistant Enterobacteriaceae
Phenotype
Carbapenems
bacteria
Water Microbiology
Bacteria
Subjects
Details
- ISSN :
- 14354373
- Volume :
- 37
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....3be3715452c51e0a1c6adcaaef83a69c