Back to Search
Start Over
Targeted disruption of the VH 81X gene: Influence on the B cell repertoire
- Source :
- European Journal of Immunology. 27:307-314
- Publication Year :
- 1997
- Publisher :
- Wiley, 1997.
-
Abstract
- We have generated a mutant mouse in which the most D-proximal V(H) gene (V(H)81X) has been disrupted by introducing a neomycin-resistance gene into the V(H)81X exon by means of gene targeting in embryonic stem cells. The mutant mice generated are unable to express the V(H)81X gene but appear to display a normal pattern of B cell differentiation as well as normal numbers of bone marrow and peripheral B cells from fetal life all through ontogeny. They mount normal immune responses to several different antigens tested. In contrast, the distribution of V(H) gene rearrangements in the V(H)7183 family is altered in homozygous mutant mice. Thus, the antibody repertoire of the targeted mice is modified, at least as far as the expression of V(H)7183 genes is concerned.
- Subjects :
- Immunology
Mutant
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Bone Marrow Cells
Biology
Immunophenotyping
Mice
Exon
Antigen
medicine
Animals
Immunology and Allergy
Gene
B cell
Mice, Knockout
B-Lymphocytes
Mice, Inbred BALB C
Genes, Immunoglobulin
Gene targeting
Embryonic stem cell
Molecular biology
Hematopoiesis
Mice, Inbred C57BL
medicine.anatomical_structure
Antibody Formation
Knockout mouse
Antibody Diversity
Subjects
Details
- ISSN :
- 15214141 and 00142980
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....3c0ff4fdb6785e8e53046dcc8a7f7cae