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Retinal expression and localization of Mef2c support its important role in photoreceptor gene expression
- Source :
- Biochemical and biophysical research communications. 483(1)
- Publication Year :
- 2016
-
Abstract
- Photoreceptor-specific gene expression is controlled by a hierarchical network of transcription factors, including the master regulators cone-rod homeobox (Crx) and neural retina leucine zipper (Nrl). Myocyte-enhancer factor 2c (Mef2c) is an ubiquitously expressed transcription factor with important functions in the cardiovascular system. Here, we performed a detailed analysis of Mef2c expression, localization and function in the retina to further elucidate its potential role for photoreceptor gene regulation. We showed that murine retinal Mef2c mRNA expression was high at birth and peaked at late postnatal developmental stages. Using immunohistochemistry and Western blot, Mef2c protein was detected in the outer nuclear layer of adult mouse and human retinas and localized to the nucleus of 661W photoreceptor-like cells. Mef2c knock-down in 661W cells reduced the expression of arrestin 3 (Arr3) and medium-wave-sensitive cone opsin (Opn1mw) but increased transcript levels of mitogen-activated protein kinase 15 (Mapk15) and phosphodiesterase 6h (Pde6h). In conclusion, Mef2c is highly expressed in the retina where it modulates photoreceptor-specific gene expression.
- Subjects :
- 0301 basic medicine
genetic structures
Arrestins
Biophysics
Biology
Biochemistry
Retina
03 medical and health sciences
Mice
0302 clinical medicine
3',5'-Cyclic-GMP Phosphodiesterases
Gene expression
OPN1MW
medicine
Animals
Humans
MEF2C
Tissue Distribution
RNA, Small Interfering
Outer nuclear layer
Extracellular Signal-Regulated MAP Kinases
Molecular Biology
Transcription factor
Regulation of gene expression
Cell Nucleus
Cyclic Nucleotide Phosphodiesterases, Type 6
MEF2 Transcription Factors
Cell Biology
Molecular biology
eye diseases
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
ARR3
sense organs
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 483
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....3c14afbee71d7b75cd0538444cbf609a