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Blastocyst-induced changes in the bovine endometrial transcriptome
- Source :
- Reproduction. 156:219-229
- Publication Year :
- 2018
- Publisher :
- Bioscientifica, 2018.
-
Abstract
- The objectives of this study were (i) to determine whether blastocyst-induced responses in endometrial explants were detectable after 6- or 24-h co-culture in vitro; (ii) to test if direct contact is required between embryos and the endometrial surface in order to stimulate endometrial gene expression; (iii) to establish the number of blastocysts required to elicit a detectable endometrial response; (iv) to investigate if upregulation of five interferon-stimulated genes (ISGs) in the endometrium was specific to the blastocyst stage and (v) to test if alterations in endometrial gene expression can be induced by blastocyst-conditioned medium. Exposure of endometrial explants to Day 8 blastocysts in vitro for 6 or 24 h induced the expression of ISGs (MX1, MX2, OAS1, ISG15, RSAD2); expression of IFNAR1, IFNAR2, NFKB1, IL1B, STAT1, LGALS3BP, LGALS9, HPGD, PTGES, ITGB1, AKR1C4, AMD1 and AQP4 was not affected. Culture of explants in the presence of more than five blastocysts was sufficient to induce the effect, with maximum expression of ISGs occurring in the presence of 20 blastocysts. This effect was exclusive to blastocyst stage embryos; oocytes, 2-cell embryos or Day 5 morulae did not alter the relative abundance of any of the transcripts examined. Direct contact between blastocysts and the endometrial surface was not required in order to alter the abundance of these transcripts and blastocyst-conditioned medium alone was sufficient to stimulate a response. Results support the notion that local embryo–maternal interaction may occur as early as Day 8 of pregnancy in cattle.
- Subjects :
- 0301 basic medicine
Embryology
Embryonic Development
Biology
Endometrium
Embryo Culture Techniques
Andrology
Transcriptome
03 medical and health sciences
Endocrinology
Downregulation and upregulation
Pregnancy
Gene expression
medicine
Animals
RNA, Messenger
Blastocyst
Obstetrics and Gynecology
Embryo
Cell Biology
ISG15
Coculture Techniques
In vitro
Up-Regulation
030104 developmental biology
medicine.anatomical_structure
Reproductive Medicine
Culture Media, Conditioned
embryonic structures
Cattle
Female
Interferons
Subjects
Details
- ISSN :
- 17417899 and 14701626
- Volume :
- 156
- Database :
- OpenAIRE
- Journal :
- Reproduction
- Accession number :
- edsair.doi.dedup.....3c2db840eb76211aa77532353991d4e7
- Full Text :
- https://doi.org/10.1530/rep-18-0188