VIM-2–producing Multidrug-Resistant Pseudomonas aeruginosa ST175 Clone, Spain

Authors :: Antonio Oliver
Francisca Sanz
Laura Barrado
Joaquín R. Otero
Jennifer Villa
M. Angeles Orellana
Carlos Juan
Fernando Chaves
Esther Viedma
Source :: Emerging Infectious Diseases, Emerging Infectious Diseases, Vol 18, Iss 8, Pp 1235-1241 (2012)
Publication Year :: 2012
Publisher :: Centers for Disease Control and Prevention, 2012.
Abstract: A total of 183 patients were colonized or infected with multidrug-resistant Pseudomonas aeruginosa isolates at a hospital in Spain during 2007-2010; prevalence increased over this period from 2.8% to 15.3%. To characterize these isolates, we performed molecular epidemiologic and drug resistance analysis. Genotyping showed that 104 (56.8%) isolates belonged to a single major clone (clone B), which was identified by multilocus sequence typing as sequence type (ST) 175. This clone was initially isolated from 5 patients in 2008, and then isolated from 23 patients in 2009 and 76 patients in 2010. PCR analysis of clone B isolates identified the b/a(VIM-2) gene in all but 1 isolate, which harbored b/a(IMP-22). ST175 isolates were susceptible to only amikacin (75%) and colistin (100%). Emergence of the ST175 clone represents a major health problem because it compromises therapy for treatment of P aeruginosa nosocomial infections.<br />This study was supported by Spanish Network for the Research in Infectious Diseases (RD06/0008) from the Instituto de Salud Carlos III, Spain.