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The impact of cesarean delivery on infant DNA methylation
- Source :
- BMC Pregnancy and Childbirth, Vol 21, Iss 1, Pp 1-8 (2021), BMC Pregnancy and Childbirth
- Publication Year :
- 2021
- Publisher :
- BMC, 2021.
-
Abstract
- Background Mounting evidence suggests that cesarean delivery may have a long-lasting effect on infant health. But the underlying mechanisms remain unclear. This study aims to examine whether cesarean delivery on maternal request without any medical indications (CDMR) impacts DNA methylation status in the umbilical cord blood of the infant. Methods A cross-sectional study was conducted in Shanghai, China. A total of 70 CDMR and 70 vaginal deliveries (VD) were recruited in 2012. The cord blood DNA methylation status was measured in 30 CDMR and 30 VD newborns using Illumina Infinium Human Methylation 450 K BeadChip. To validate the results, the cord blood DNA methylation status was measured in another 40 CDMR and 40 VD newborns using targeted bisulfite sequencing assay. A total of 497 CpG sites from 40 genes were included in the analysis. Results A total of 165 differentially methylated positions (DMPs) exhibited differences in DNA methylation by 10% or more between the CDMR and VD groups, many of which were related to the development of the immune system. Based on the targeted bisulfite sequencing assay, 16 genes (16/22, 72.7%) had higher methylation level in the CDMR group than the VD group. Among them, 5 genes were related to the immune system. After considering the estimation of cell type proportions, there was few significant differences in DNA methylation between CDMR and VD groups. Conclusions The DMPs identified between CDMR and VD groups might be largely explained by the cell type proportions. Further studies are needed to examine DNA methylation in each cell type separately.
- Subjects :
- Adult
Male
China
Bisulfite sequencing
Gestational Age
Umbilical cord
Perinatal epidemiology
lcsh:Gynecology and obstetrics
Epigenesis, Genetic
Andrology
03 medical and health sciences
0302 clinical medicine
Immune system
Pregnancy
Humans
Medicine
Gene
lcsh:RG1-991
030304 developmental biology
0303 health sciences
DNA methylation
Cesarean Section
business.industry
Infant, Newborn
Obstetrics and Gynecology
Cord blood
DNA
Methylation
Fetal Blood
Cross-Sectional Studies
medicine.anatomical_structure
Cesarean deliveries on maternal request
CpG site
030220 oncology & carcinogenesis
CpG Islands
Female
business
Research Article
Maternal Age
Subjects
Details
- Language :
- English
- ISSN :
- 14712393
- Volume :
- 21
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Pregnancy and Childbirth
- Accession number :
- edsair.doi.dedup.....3c5dff467dd9f73b18fbf81ff63fe299