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Antiprostate Cancer Activity of Ineupatolide Isolated from Carpesium cernuum L
- Source :
- BioMed Research International, BioMed Research International, Vol 2021 (2021)
- Publication Year :
- 2021
- Publisher :
- Hindawi, 2021.
-
Abstract
- Objective. The aim of the study was to investigate the antiprostate cancer effects and mechanism of ineupatolide (T-21), a natural product isolated from the Compositae plant Carpesium cernuum L., on PC-3 human prostate cancer cells. Methods. The effect of T-21 on the proliferation of PC-3 cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, cell migration, and invasion experiments; the morphology of cell apoptosis was observed by Hoechst-propidium iodide staining; the effects of T-21 on PC-3 cell apoptosis and the cell cycle were evaluated by flow cytometry; and the effect of T-21 on the expression levels of phosphorylated protein kinase B (p-AKT), AKT, X-linked inhibitor of apoptosis protein (xlAP), procaspase-3, and poly (ADP-ribose) polymerase (PARP) in PC-3 cells was measured by western blotting. Results. T-21 significantly inhibited the proliferation of cells, and its half-maximal inhibitory concentrations at 12, 24, and 48 h were 38.46 ± 1.01 , 24.63 ± 0.70 , and 7.36 ± 0.58 μ M , respectively. T-21 may promote cell apoptosis in a concentration-dependent manner and block the cell cycle in the G2 and S phases. In addition, T-21 significantly reduced the protein expression levels of p-AKT, AKT, xlAP, procaspase-3, and PARP. Conclusion. T-21 exhibits antiproliferation effects on PC-3 cells by promoting apoptosis and arresting the cell cycle in the G2 and S phases. The possible mechanism underlying its potential therapeutic effects against prostate cancer is related to the AKT/xlAP pathway.
- Subjects :
- Male
0301 basic medicine
Article Subject
Phytochemicals
Apoptosis
X-Linked Inhibitor of Apoptosis Protein
Asteraceae
Inhibitor of apoptosis
General Biochemistry, Genetics and Molecular Biology
Flow cytometry
03 medical and health sciences
0302 clinical medicine
medicine
Humans
Protein kinase B
Cell Proliferation
General Immunology and Microbiology
medicine.diagnostic_test
Chemistry
Prostatic Neoplasms
Cancer
Cell migration
General Medicine
Cell cycle
medicine.disease
Antineoplastic Agents, Phytogenic
Molecular biology
030104 developmental biology
030220 oncology & carcinogenesis
PC-3 Cells
Cancer cell
Medicine
Proto-Oncogene Proteins c-akt
Signal Transduction
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....3c6568b13d683f5706a5b938dfd15045
- Full Text :
- https://doi.org/10.1155/2021/5515961