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Validated UPLC and TLC-Densitometry Stability Indicating Methods for the Determination of Rafoxanide in the Presence of Its Degradation Products
- Source :
- Journal of chromatographic science. 54(9)
- Publication Year :
- 2016
-
Abstract
- Two sensitive and accurate stability-indicating chromatographic methods were developed and validated for the determination of rafoxanide (RFX). Degradation products were identified by mass spectrometry and IR spectroscopy. The first is ultra-performance liquid chromatography method where separation was performed using acetonitrile:0.005 M potassium dihydrogen orthophosphate (pH 3.5) in a ratio of 80:20 by volume as a mobile phase using a Hypersil GOLD™ C8 column 1.9 mm (50 × 2.1 mm), UV detection was adjusted at 220 nm and the flow rate was 0.6 mL min-1. The other is a thin-layer chromatography-densitometry method where separation was achieved using a mobile phase composed of chloroform:ethyl acetate:toluene:ammonia (5:4:3:0.1 by volume) on silica gel 60 F254 plates, and densitometric detection was done at 280 nm. Validation was achieved as per the ICH guidelines. The proposed methods proved to be accurate, robust, specific and suitable for application as stability-indicating methods for routine analysis of RFX in quality control laboratories.
- Subjects :
- Chloroform
Chromatography
Silica gel
010401 analytical chemistry
Ethyl acetate
General Medicine
Mass spectrometry
030226 pharmacology & pharmacy
01 natural sciences
High-performance liquid chromatography
0104 chemical sciences
Analytical Chemistry
03 medical and health sciences
Rafoxanide
chemistry.chemical_compound
0302 clinical medicine
chemistry
Acetonitrile
Densitometry
Subjects
Details
- ISSN :
- 1945239X
- Volume :
- 54
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Journal of chromatographic science
- Accession number :
- edsair.doi.dedup.....3c819fe43b9bd1ddb881cf5f0488543d