STUDIES WITH A SIMPLIFIED NITROPRUSSIDE TEST FOR KETONE BODIES IN URINE, SERUM, PLASMA, AND MILK

A standardized dip-and-read test for the detection of ketone bodies is described. This simplified nitroprusside test has a reading time of only 15 sec. The test is of value in assessing the severity of human ketosis since it can be used to determine the level of ketone bodies in both urine and serum. Testing urine from small experimental animals and testing cow's milk for early detection of bovine ketosis are easily accomplished with the stick test. The sensitivity and reactivity of the test appear to be the same in all body fluids studied. Data are presented which establish the accuracy, the reliability, and the utility of the stick test as a tool in scientific investigations.