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Insights into the antibacterial mechanism of PEGylated nano-bacitracin A against Streptococcus pneumonia: both penicillin-sensitive and penicillin-resistant strains
- Source :
- International Journal of Nanomedicine
- Publication Year :
- 2018
- Publisher :
- Dove Medical Press, 2018.
-
Abstract
- Wei Hong, Lipeng Liu, Zehui Zhang, Yining Zhao, Dexian Zhang, Mingchun Liu Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning Province 110866, People’s Republic of China Background: Multidrug-resistant (MDR) Streptococcus pneumonia constitute a major worldwide public health concern. Materials and methods: In our preliminary study, PEGylated nano-self-assemblies of bacitracin A (PEGylated Nano-BA12K) showed strong antibacterial potency against reference S. pneumonia strain (ATCC 49619). In this study, the possibility of applying PEGylated Nano-BA12K against penicillin-resistant S. pneumonia was further investigated. In addition, the underlying antibacterial mechanism of PEGylated Nano-BA12K against both sensitive and resistant S. pneumonia was also clarified systematically, since S. pneumonia was naturally resistant to its unassembled counterpart bacitracin A (BA). Results: PEGylated Nano-BA12K showed strong antibacterial potency against 13 clinical isolates of S. pneumonia, including five penicillin-resistant strains. Structural changes, partial collapse, and even lysis of both penicillin-sensitive and penicillin-resistant bacteria were observed after incubation with PEGylated Nano-BA12K via transmission electron microscopy and atomic force microscopy. Thus, the cell wall or/and cell membrane might be the main target of PEGylated Nano-BA12K against S. pneumonia. PEGylated Nano-BA12K exhibited limited effect on the permeabilization and peptidoglycan content of cell wall. Surface pressure measurement suggested that PEGylated Nano-BA12K was much more tensioactive than BA, which was usually translated into a good membranolytic effect, and is helpful to permeabilize the cell membrane and damage membrane integrity, as evidenced by depolarization of the membrane potential, permeabilization of membrane and leakage of calcein from liposomes. Conclusion: Collectively, great cell membrane permeability and formidable membrane disruption may work together for the strong antibacterial activity of PEGylated Nano-BA12K against S. pneumonia. Taken together, PEGylated Nano-BA12K has excellent potential against both penicillin-sensitive and penicillin-resistant S. pneumonia and might be suitable for the treatment of S. pneumonia infectious diseases. Keywords: PEGylated Nano-BA12K, multidrug-resistant, Streptococcus pneumonia, penicillin-sensitive and penicillin-resistantCorrigendumfor this paper has been published
- Subjects :
- 0301 basic medicine
Streptococcus pneumonia
Cell Membrane Permeability
Lysis
PEGylated Nano-BA12K
Lipid Bilayers
Pharmaceutical Science
02 engineering and technology
medicine.disease_cause
Membrane Potentials
Polyethylene Glycols
Cell membrane
chemistry.chemical_compound
Polylactic Acid-Polyglycolic Acid Copolymer
International Journal of Nanomedicine
Drug Discovery
Original Research
Streptococcus
General Medicine
021001 nanoscience & nanotechnology
Anti-Bacterial Agents
Streptococcus pneumoniae
medicine.anatomical_structure
0210 nano-technology
Antibacterial activity
medicine.drug
Penicillin Resistance
multidrug-resistant
Biophysics
Bioengineering
Microbial Sensitivity Tests
Penicillins
Peptidoglycan
Bacitracin
Buffers
Microbiology
Biomaterials
03 medical and health sciences
Drug Resistance, Bacterial
medicine
Humans
Surface Tension
Organic Chemistry
technology, industry, and agriculture
penicillin-sensitive and penicillin-resistant
Multiple drug resistance
Penicillin
030104 developmental biology
chemistry
Nanoparticles
Subjects
Details
- Language :
- English
- ISSN :
- 11782013 and 11769114
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- International Journal of Nanomedicine
- Accession number :
- edsair.doi.dedup.....3ca707442d264b89c5f9db8d876f7a0f