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Duodenal acidification induces gastric relaxation and alters epithelial barrier function by a mast cell independent mechanism
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020), Dipòsit Digital de Documents de la UAB, Universitat Autònoma de Barcelona, Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group, 2020.
-
Abstract
- Duodenal hyperpermeability and low-grade inflammation in functional dyspepsia is potentially related to duodenal acid exposure. We aimed to evaluate in healthy volunteers the involvement of mast cell activation on the duodenogastric reflex and epithelial integrity during duodenal acidification. This study consisted of 2 parts: (1) Duodenal infusion of acid or saline during thirty minutes in a randomized, double-blind cross-over manner with measurement of intragastric pressure (IGP) using high resolution manometry and collection of duodenal biopsies to measure epithelial barrier function and the expression of cell-to-cell adhesion proteins. Mast cells and eosinophils were counted and activation and degranulation status were assessed. (2) Oral treatment with placebo or mast cell stabilizer disodiumcromoglycate (DSCG) prior to duodenal perfusion with acid, followed by the procedures described above. Compared with saline, acidification resulted in lower IGP (P
- Subjects :
- Male
0301 basic medicine
Functional dyspepsia
DYSPEPSIA
SYMPTOMS
Biopsy
medicine.medical_treatment
lcsh:Medicine
Cell Degranulation
Epithelium
Mice
0302 clinical medicine
HISTAMINE
Mast Cells
Mast cell stabilizer
lcsh:Science
Saline
HYPERSENSITIVITY
Cross-Over Studies
Multidisciplinary
biology
Chemistry
Stomach
Degranulation
Hydrogen-Ion Concentration
Mast cell
Healthy Volunteers
Multidisciplinary Sciences
ACID-SECRETION
medicine.anatomical_structure
Science & Technology - Other Topics
Female
030211 gastroenterology & hepatology
Saline Solution
medicine.symptom
Perfusion
Adult
medicine.medical_specialty
Duodenum
medicine.drug_class
DISORDERS
INHIBITION
Inflammation
Tryptase
Article
Permeability
03 medical and health sciences
Double-Blind Method
Internal medicine
Cromolyn Sodium
Cell Adhesion
Pressure
medicine
Animals
Humans
EXPOSURE
Electrodes
Tight junctions
Science & Technology
lcsh:R
Small intestine
Translational research
030104 developmental biology
Endocrinology
MUCOSAL PERMEABILITY
Reflex
biology.protein
RAT
lcsh:Q
Acids
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....3ca88dbdc81645a5f1923730b5dc079e