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Enhancing Carbohydrate Productivity of Chlorella sp. AE10 in Semi-continuous Cultivation and Unraveling the Mechanism by Flow Cytometry
- Source :
- Applied biochemistry and biotechnology. 185(2)
- Publication Year :
- 2017
-
Abstract
- Accumulated carbohydrate in microalgae is promising feedstock for bioethanol fermentation. Selection of suitable cultivation conditions in semi-continuous cultivation is critical to achieve a high carbohydrate productivity. In the current study, the effects of macro-nutrient (nitrogen, phosphorus, and sulfur) limitations and light intensity were evaluated for the carbohydrate accumulations of Chlorella sp. AE10 under 10% CO2 conditions. It was shown that nitrogen limitation and high light intensity were effective for improving carbohydrate productivity. The average carbohydrate and biomass productivity in semi-continuous cultivation with 1/4 N medium and 1000 μmol photons m-2 s-1 was 0.673 and 0.93 g L-1 day-1, respectively. Sulfur and phosphorus limitations could improve the carbohydrate content but they could not enhance the carbohydrate productivity. The cell cycle progression and chlorophyll a were investigated using flow cytometry (FCM). The results showed that macro-nutrient limitation and high light intensity indeed influenced cell cycle progression and led to the formation of polyploid cells along with the carbohydrate accumulation in a certain range. FCM was rapid and accurate method to investigate the operation conditions why 1/4 N, 2 days as a cycle, and high light intensity were optimal ones. In addition, the remaining high level of photosynthesis activity was also important for achieving a high carbohydrate productivity. Dynamic tracking of carbohydrate accumulation is helpful for establishment of a semi-continuous cultivation for enhancing carbohydrate productivity in microalgae.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Chlorophyll
Biomass
Bioengineering
Chlorella
Carbohydrate metabolism
Photosynthesis
01 natural sciences
Applied Microbiology and Biotechnology
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
010608 biotechnology
Botany
Food science
Molecular Biology
biology
Cell Cycle
General Medicine
Carbohydrate
biology.organism_classification
Flow Cytometry
Light intensity
030104 developmental biology
chemistry
Carbohydrate Metabolism
Fermentation
Biotechnology
Subjects
Details
- ISSN :
- 15590291
- Volume :
- 185
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Applied biochemistry and biotechnology
- Accession number :
- edsair.doi.dedup.....3cc039dfedd4435d3775492ec2f09861