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Therapeutic properties of Scutellaria baicalensis in db/db mice evaluated using Connectivity Map and network pharmacology
- Source :
- SCIENTIFIC REPORTS(7), Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group, 2017.
-
Abstract
- We have reported that an extract of Scutellaria baicalensis (ESB) has effects against obesity and hypertriglyceridemia in type 2 diabetic animal model (db/db mouse). In the present study, we tried to explain the pharmacological effects of ESB by integrating gene expression information from db/db mouse liver with that of ESB-treated HepG2 hepatocellular carcinoma cells. Using Connectivity Map (cmap) analysis, we found an inverse relationship in the pharmaceutical profiles based on gene expression between db/db mouse liver and ESB-treated HepG2 cells. This inverse relationship between the two data sets was also observed for pathway activities. Functional network analysis showed that biological functions associated with diabetes and lipid metabolism were commonly enriched in both data sets. We also observed a similarity in distribution of cmap enrichment scores between db/db mouse liver and human diabetic liver, whereas there was an inverse pattern of cmap enrichment scores in human diabetic liver compared with ESB-treated HepG2 cells. This relationship might explain the pharmacological activities of ESB against db/db mouse and possible effectiveness of ESB against human diabetes. We expect that our approach using in vitro cell lines could be applied in predicting the pharmacological effectiveness of herbal drugs in in vivo systems.
- Subjects :
- Blood Glucose
Male
0301 basic medicine
Mice, Transgenic
Pharmacology
Article
Transcriptome
Mice
03 medical and health sciences
0302 clinical medicine
In vivo
Diabetes mellitus
Gene expression
Diabetes Mellitus
medicine
Animals
Humans
Gene Regulatory Networks
Multidisciplinary
biology
Plant Extracts
Gene Expression Profiling
Computational Biology
Lipid metabolism
Hep G2 Cells
Lipid Metabolism
biology.organism_classification
medicine.disease
Gene expression profiling
Gene Ontology
030104 developmental biology
Gene Expression Regulation
Liver
Cell culture
030220 oncology & carcinogenesis
Scutellaria baicalensis
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....3cd0ffe18a3465237a07dd08894a097d
- Full Text :
- https://doi.org/10.1038/srep41711