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Bilateral muscle fiber and nerve influences by TNF-alpha in response to unilateral muscle overuse : studies on TNF receptor expressions
- Source :
- BMC Musculoskeletal Disorders, BMC Musculoskeletal Disorders, Vol 18, Iss 1, Pp 1-17 (2017)
- Publication Year :
- 2017
- Publisher :
- Umeå universitet, Institutionen för integrativ medicinsk biologi (IMB), 2017.
-
Abstract
- Background TNF-alpha is suggested to be involved in muscle damage and muscle inflammation (myositis). In order to evaluate whether TNF-alpha is involved in the myositis that occurs in response to muscle overuse, the aim was to examine the expression patterns of TNF receptors in this condition. Methods A rabbit muscle overuse model leading to myositis in the soleus muscle was used. The expression patterns of the two TNF receptors Tumor Necrosis Factor Receptor type 1 (TNFR1) and Tumor Necrosis Factor Receptor type 2 (TNFR2) were investigated. In situ hybridization and immunofluorescence were utilized. Immunostainings for desmin, NK-1R and CD31 were made in parallel. Results Immunoreactions (IR) for TNF receptors were clearly observed in white blood cells, fibroblasts and vessel walls, and most interestingly also in muscle fibers and nerve fascicles in the myositis muscles. There were very restricted reactions for these in the muscles of controls. The upregulation of TNF receptors was for all types of structures seen for both the experimental side and the contralateral nonexperimental side. TNF receptor expressing muscle fibers were present in myositis muscles. They can be related to attempts for reparation/regeneration, as evidenced from results of parallel stainings. Necrotic muscle fibers displayed TNFR1 mRNA and TNFR2 immunoreaction (IR) in the invading white blood cells. In myositis muscles, TNFR1 IR was observed in both axons and Schwann cells while TNFR2 IR was observed in Schwann cells. Such observations were very rarely made for control animals. Conclusions The findings suggest that there is a pronounced involvement of TNF-alpha in the developing myositis process. Attempts for reparation of the muscle tissue seem to occur via both TNFR1 and TNFR2. As the myositis process also occurs in the nonexperimental side and as TNF receptors are confined to nerve fascicles bilaterally it can be asked whether TNF-alpha is involved in the spreading of the myositis process to the contralateral side via the nervous system. Taken together, the study shows that TNF-alpha is not only associated with the inflammation process but that both the muscular and nervous systems are affected and that this occurs both on experimental and nonexperimental sides.
- Subjects :
- 0301 basic medicine
Nervous system
Muscle tissue
Pathology
medicine.medical_specialty
lcsh:Diseases of the musculoskeletal system
Medicinska och farmaceutiska grundvetenskaper
Cumulative Trauma Disorders
Muscle Fibers, Skeletal
Fluorescent Antibody Technique
In situ hybridization
nerve structures
03 medical and health sciences
Nerve Fibers
0302 clinical medicine
Rheumatology
medicine
Animals
Humans
Receptors, Tumor Necrosis Factor, Type II
Orthopedics and Sports Medicine
RNA, Messenger
Receptor
Myositis
Soleus muscle
Tumor Necrosis Factor-alpha
business.industry
Nerve structures
Basic Medicine
medicine.disease
Up-Regulation
TNFR1
Disease Models, Animal
TNFR2
030104 developmental biology
medicine.anatomical_structure
Receptors, Tumor Necrosis Factor, Type I
Muscle
Female
Desmin
Tumor necrosis factor alpha
Rabbits
lcsh:RC925-935
business
030217 neurology & neurosurgery
Research Article
TNF-alpha
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- BMC Musculoskeletal Disorders, BMC Musculoskeletal Disorders, Vol 18, Iss 1, Pp 1-17 (2017)
- Accession number :
- edsair.doi.dedup.....3cdd12053aa6e76e17861e38e937f15f