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Burkholderia denitrificans sp. nov., isolated from the soil of Dokdo Island, Korea
- Source :
- Journal of microbiology (Seoul, Korea). 50(5)
- Publication Year :
- 2011
-
Abstract
- A novel, Gram-negative, bacterial strain KIS30-44(T) was identified from wet forest soil collected on the Korean island of Dokdo. Growth of the strain was observed at 15-30°C, pH 5-9, 0-3% NaCl, and 950 mM KNO(3). KIS30-44(T) reduced nitrate to nitrogen gas. Analysis of the 16S rRNA gene sequence showed that KIS30-44(T) was phylogenetically related to Burkholderia sacchari, Burkholderia mimosarum, and Burkholderia oxyphila (98.1%, 98.0%, and 98.0% sequence similarity, respectively). The genomic G+C content was 63.5 mol%. KIS30-44(T) exhibited less than 52% DNA-DNA relatedness with the type strains of 9 closely related Burkholderia species. The major isoprenoid quinone was Q-8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminolipids. The major fatty acids in KIS30-44(T) were C(16:0), C(18:1) ω7c and summed feature 3 (iso-C(15:0) 2-OH and C(16:1) ω7c), and the strain contained half the amount of C(17:0) cyclo found in the 9 closely related Burkholderia species. The results of these phenotypic, 16S rRNA gene sequence, DNA-DNA hybridization, and chemotaxonomic data indicate that KIS30-44(T) represents a novel species within the genus Burkholderia, for which the name Burkholderia denitrificans (Type strain KIS30-44(T) =KACC 12733(T) =DSM 24336(T)) is proposed.
- Subjects :
- DNA, Bacterial
Burkholderia
Molecular Sequence Data
Burkholderia denitrificans
Applied Microbiology and Biotechnology
Microbiology
chemistry.chemical_compound
RNA, Ribosomal, 16S
Botany
Nitrogen gas
Republic of Korea
Phylogeny
Soil Microbiology
Phosphatidylglycerol
Base Composition
biology
Fatty Acids
Burkholderia oxyphila
General Medicine
biology.organism_classification
16S ribosomal RNA
Terpenoid
chemistry
Burkholderia sacchari
Taxonomy (biology)
Subjects
Details
- ISSN :
- 19763794
- Volume :
- 50
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of microbiology (Seoul, Korea)
- Accession number :
- edsair.doi.dedup.....3d31d63679fe65d9d5ad5dce308482ea