Back to Search
Start Over
Image-guided surgery of head and neck carcinoma in rabbit models by intra-operatively defining tumour-infiltrated margins and metastatic lymph nodes
- Source :
- EBioMedicine
- Publication Year :
- 2019
- Publisher :
- Elsevier, 2019.
-
Abstract
- Background The infiltrative nature and lymphatic metastasis of head and neck squamous cell carcinoma (HNSCC) are the main reasons leading to its poor prognosis. Methods A multimodal surface-enhanced resonance Raman spectroscopy (SERRS) and magnetic resonance (MR) nanoprobe, in which paramagnetic chelators and heptamethine cyanine-based Raman reporter molecules were functionalized on a gold nanostar (AuS) surface was developed. Preoperative MRI and intraoperative SERRS-guided surgery were performed on rabbits bearing head and neck VX2 tumours to determine feasibility of the MR/SERRS probe in defining tumour marginal infiltration and lymph nodes metastasis. Findings Preoperative T1-weighted MRI (T1W-MRI) unambiguously delineated the orthotopic head and neck VX2 tumour xenograft and detected the metastatic lymph nodes in rabbit models after intravenous administration of the probe. With the assistance of a hand-held Raman detector, the probe not only intra-operatively demarcated invasive tumour margins but also successfully distinguished metastatic lymph nodes via a remarkable attenuated Raman signal. Importantly, the group of rabbits subjected to the SERRS-guided surgery exhibited prolonged median survival time (78 days) compared with that of the control group without surgical intervention (29 days) or the group treated with conventional white-light-guided surgery (42 days) (P Interpretation we developed a novel AuS-based multimodal MR/SERRS probe. The capability of this probe to identify both a tumour xenograft and metastatic lymph nodes preoperatively by MRI and intra-operatively by SERRS not only avoids the need for unnecessary resection of neurological structures but also provides a new opportunity to improve the surgical prognosis of head and neck carcinoma of infiltrative nature.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Poor prognosis
Research paper
Nanoprobe
Spectrum Analysis, Raman
General Biochemistry, Genetics and Molecular Biology
Metastasis
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
medicine
Surface-enhanced resonance Raman scattering (SERRS)
Animals
Humans
Neoplasm Invasiveness
Magnetic resonance imaging (MRI)
Head and neck carcinoma
Neoplasm Staging
Lymph node metastasis
medicine.diagnostic_test
business.industry
Carcinoma
Head and neck squamous cell carcinoma (HNSCC)
Margins of Excision
Magnetic resonance imaging
General Medicine
medicine.disease
Head and neck squamous-cell carcinoma
Magnetic Resonance Imaging
Image-guided resection
Disease Models, Animal
030104 developmental biology
Image-guided surgery
Treatment Outcome
Surgery, Computer-Assisted
Head and Neck Neoplasms
030220 oncology & carcinogenesis
Lymphatic Metastasis
Molecular Probes
Radiology
Lymph
Rabbits
business
Subjects
Details
- Language :
- English
- ISSN :
- 23523964
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- EBioMedicine
- Accession number :
- edsair.doi.dedup.....3d54a89a8cfa1f26f3121656b74b4da9