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Design, synthesis, physical and chemical characterisation, and biological interactions of lectin-targeted latex nanoparticles bearing Gd-DTPA chelates: an exploration of magnetic resonance molecular imaging (MRMI)
- Source :
- Histochemistry and cell biology. 123(3)
- Publication Year :
- 2005
-
Abstract
- The physical and chemical parameters involved in the design and synthesis of biospecifically targeted nanoparticulate contrast media for magnetic resonance molecular imaging (MRMI) were explored in this pilot investigation. Latex nanoparticles 100, 400 and 900 nm in diameter were doubly derivatised, first with tomato lectin and then with gadolinium(III)-diethylenetriamine pentaacetic acid (Gd-chelates) to target them to epithelial and endothelial glycocalyceal N-glycans and to generate contrast enhancement in magnetic resonance imaging (MRI). After intravenous injection into mice, human placental cotyledons or human Vena saphena magna, contrasty images of the vascular structures were obtained in 1.5 T MRI with spatial resolution 0.1 mm in the imaging plane and 0.6 mm in the z axis, persisting >60 min and resistant to washing out by buffer rinses. Ultrastructural analysis of the nanoparticles revealed the targeting groups at the nanoparticle surfaces and the distribution of the Gd-chelates within the nanoparticles and enabled counts for use in determining relaxivity. The relaxivity values revealed were extremely high, accounting for the strong MR signals observed. Occasionally, nanoparticles larger than 100 nm were seen in close spatial association with disrupted regions of cell membrane or of collagen fibrils in the extracellular matrix. The data suggest that 100-nm nanoparticles generate adequate contrast for MRMI and cause least disruption to endothelial cell surfaces.
- Subjects :
- Gadolinium DTPA
Histology
Magnetic Resonance Spectroscopy
Gadolinium
Nanoparticle
chemistry.chemical_element
Contrast Media
In Vitro Techniques
Cell membrane
Mice
Nuclear magnetic resonance
medicine
Animals
Humans
Chelation
Molecular Biology
medicine.diagnostic_test
biology
Chemistry
Lectin
Magnetic resonance imaging
Cell Biology
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Imaging
Microspheres
Medical Laboratory Technology
medicine.anatomical_structure
biology.protein
Microscopy, Electron, Scanning
Blood Vessels
Polystyrenes
Molecular imaging
Plant Lectins
Subjects
Details
- ISSN :
- 09486143
- Volume :
- 123
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Histochemistry and cell biology
- Accession number :
- edsair.doi.dedup.....3d84cea710a52e9198c6fdb6c55497ce