Effect of a short-term captopril treatment on serum and tissue angiotensin I-converting enzyme activity from four mammalian species. Differences using diamide in the in vitro assay

1. Angiotensin I-converting enzyme (ACE) activity was determined in serum and nine tissues from control and captopril-treated rats, mice, guinea pigs and rabbits. 2. ACE activity was determined with and without sample pretreatment with diamide (total and basal activity, respectively). 3. A very different pattern of response to captopril was observed among the different species. 4. There was no relationship between serum ACE activity and the response to captopril. 5. There were important differences in the determinations of total or basal ACE activities. 6. Endogenous ACE inhibitors were found in some tissues from mouse and rabbit.