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Oligonucleotides in human urine do not contain 8-oxo-7,8-dihydrodeoxyguanosine
- Source :
- Free Radical Biology and Medicine. 36:1378-1382
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- The promutagenic DNA modification 8-oxo-7,8-dihydrodeoxyguanosine is the most frequently used marker for oxidative stress to DNA. The unmodified base and nucleoside and the 8-hydroxylated guanine base and nucleoside are found in urine, the latter used as a global measure of oxidative stress to DNA. Nucleotide excision repair (NER) excises a 27- to 29-mer oligonucleotide with oxidative lesions, and if found in urine, it could be used as a measure of DNA repair in vivo. Enzymatic hydrolysis of human urines followed by HPLC–tandem mass spectrometry was not able to reveal oligonucleotides and/or mononucleotides with the 8-oxo-7,8-dihydrodeoxyguanosine modification. The recovery of a synthetic oligonucleotide with the modification was complete (95% confidence limits: 98–124%). These experiments show that oligonucleotides are excreted into urine, but that 8-oxo-7,8-dihydrodeoxyguanosine is found only as the mononucleoside and is not present in any significant amounts in oligonucleotides. We conclude that oligonucleotides are excreted into urine, and they do not contain oxidized lesions. Either NER products are degraded after excision or NER functions differently in vivo in humans compared with cellular systems.
- Subjects :
- Oligonucleotide
DNA repair
Guanine
Oligonucleotides
Deoxyguanosine
medicine.disease_cause
Biochemistry
Molecular biology
Mass Spectrometry
Oxidative Stress
chemistry.chemical_compound
chemistry
8-Hydroxy-2'-Deoxyguanosine
In vivo
Physiology (medical)
medicine
Humans
Nucleoside
Chromatography, High Pressure Liquid
DNA
Oxidative stress
Nucleotide excision repair
Subjects
Details
- ISSN :
- 08915849
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Free Radical Biology and Medicine
- Accession number :
- edsair.doi.dedup.....3dbe39b9d1420ed46fa20b8fc42b5562
- Full Text :
- https://doi.org/10.1016/j.freeradbiomed.2004.02.070