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Effects of temozolomide on U87MG glioblastoma cell expression of CXCR4, MMP2, MMP9, VEGF, anti-proliferatory cytotoxic and apoptotic properties
- Source :
- Molecular biology reports. 47(2)
- Publication Year :
- 2019
-
Abstract
- Temozolomide is an alkylating agent which is used in glioblastoma treatment. We aimed to investigate the effects of different concentrations of temozolomide and exposure time on U87MG glioblastoma cell expression of CXCR4, MMP2, MMP9 and VEGF. U87MG cells were cultured in different temozolomide concentrations and incubation time and the effects of temozolomide on inducing apoptosis was investigated. The levels of VEGF and CXCR4 expression were measured by RT-PCR and flowcytometry. Moreover, MMP2 and MMP9 activity and expression were assessed by ELISA and zymography. CXCR4 and VEGF expression levels decreased upon applying higher concentration of temozolomide. MMP2 and MMP-9 had lower activity in cells with longer exposure time or higher doses of temozolomide. Temozolomide induces the apoptosis in U87MG glioblastoma cells at therapeutic or higher dose. It is capable of decreasing their expression levels of VEGF and CXCR4.
- Subjects :
- 0301 basic medicine
Vascular Endothelial Growth Factor A
Receptors, CXCR4
MMP2
Cell Survival
Apoptosis
Enzyme-Linked Immunosorbent Assay
MMP9
CXCR4
Incubation period
Immunophenotyping
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
Genetics
medicine
Temozolomide
Cytotoxic T cell
Humans
Zymography
Molecular Biology
Antineoplastic Agents, Alkylating
Dose-Response Relationship, Drug
Chemistry
General Medicine
Immunohistochemistry
Gene Expression Regulation, Neoplastic
030104 developmental biology
Matrix Metalloproteinase 9
030220 oncology & carcinogenesis
Cancer research
Matrix Metalloproteinase 2
Glioblastoma
Reactive Oxygen Species
Oxidation-Reduction
Biomarkers
medicine.drug
Subjects
Details
- ISSN :
- 15734978
- Volume :
- 47
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Molecular biology reports
- Accession number :
- edsair.doi.dedup.....3dd3f409d76893614b5eeff9e5229615