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Supramolecular arrangement of protein in nanoparticle structures predicts nanoparticle tropism for neutrophils in acute lung inflammation
- Publication Year :
- 2022
- Publisher :
- Springer Science and Business Media LLC, 2022.
-
Abstract
- This study shows that the supramolecular arrangement of proteins in nanoparticle structures predicts nanoparticle accumulation in neutrophils in acute lung inflammation (ALI). We observed homing to inflamed lungs for a variety of nanoparticles with agglutinated protein (NAPs), defined by arrangement of protein in or on the nanoparticles via hydrophobic interactions, crosslinking and electrostatic interactions. Nanoparticles with symmetric protein arrangement (for example, viral capsids) had no selectivity for inflamed lungs. Flow cytometry and immunohistochemistry showed NAPs have tropism for pulmonary neutrophils. Protein-conjugated liposomes were engineered to recapitulate NAP tropism for pulmonary neutrophils. NAP uptake in neutrophils was shown to depend on complement opsonization. We demonstrate diagnostic imaging of ALI with NAPs; show NAP tropism for inflamed human donor lungs; and show that NAPs can remediate pulmonary oedema in ALI. This work demonstrates that structure-dependent tropism for neutrophils drives NAPs to inflamed lungs and shows NAPs can detect and treat ALI.
- Subjects :
- Lipopolysaccharides
Male
Agglutination
Neutrophils
Static Electricity
Biomedical Engineering
Bioengineering
Inflammation
Antibodies
Flow cytometry
mental disorders
medicine
Animals
Humans
Tissue Distribution
General Materials Science
Electrical and Electronic Engineering
Lung
Tropism
Tomography, Emission-Computed, Single-Photon
Liposome
medicine.diagnostic_test
Chemistry
fungi
Proteins
Dextrans
Opsonin Proteins
respiratory system
Condensed Matter Physics
Atomic and Molecular Physics, and Optics
respiratory tract diseases
Cell biology
Mice, Inbred C57BL
Nap
Antibody opsonization
Cross-Linking Reagents
medicine.anatomical_structure
Acute Disease
Liposomes
Nanoparticles
Muramidase
medicine.symptom
Tomography, X-Ray Computed
psychological phenomena and processes
Homing (hematopoietic)
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....3de3c7e5e03827014748aaac9cf92e29