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Solution NMR Structure of Dsy0195 Homodimer from Desulfitobacterium hafniense: First Structure Representative of the YabP Domain Family of Proteins Involved in Spore Coat Assembly
- Publication Year :
- 2011
-
Abstract
- Protein domain family YabP (PF07873) is a family of small protein domains that are conserved in a wide range of bacteria and involved in spore coat assembly during the process of sporulation. The 62-residue fragment of Dsy0195 from Desulfitobacterium hafniense, which belongs to the YabP family, exists as a homodimer in solution under the conditions used for structure determination using NMR spectroscopy. The structure of the Dsy0195 homodimer contains two identical 62-residue monomeric subunits, each consisting of five anti-parallel beta strands (β1, 23–29; β2, 31–38; β3, 41–46; β4, 49–59; β5, 69–80). The tertiary structure of the Dsy0195 monomer adopts a cylindrical fold composed of two beta sheets. The two monomer subunits fold into a homodimer about a single C2 symmetry axis, with the interface composed of two anti-parallel beta strands, β1–β1′ and β5b–β5b′, where β5b refers to the C-terminal half of the bent β5 strand, without any domain swapping. Potential functional regions of the Dsy0195 structure were predicted based on conserved sequence analysis. The Dsy0195 structure reported here is the first representative structure from the YabP family.
- Subjects :
- Models, Molecular
Protein Folding
Protein Conformation
Protein domain
Beta sheet
Desulfitobacterium
Biochemistry
Article
Protein Structure, Secondary
Structural genomics
Conserved sequence
Protein structure
Bacterial Proteins
Structural Biology
Genetics
Amino Acid Sequence
Nuclear Magnetic Resonance, Biomolecular
Spores, Bacterial
biology
Desulfitobacterium hafniense
General Medicine
biology.organism_classification
Protein tertiary structure
Protein Structure, Tertiary
Crystallography
Protein folding
Protein Multimerization
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....3dfcfb83e229fede98e7d0451beddcf3