Back to Search Start Over

Benchmarking a luciferase complementation assay for detecting protein complexes

Authors :
Johann Pellet
Patricia Cassonnet
Frédéric Tangy
Vincent Lotteau
Yves Jacob
Caroline Demeret
Mandy Muller
Louis Jones
Françoise Vuillier
Caroline Rolloy
Michel Favre
Pierre-Olivier Vidalain
Thibault Chantier
Guillaume Gaud
Gregory Neveu
Génétique, Papillomavirus et Cancer Humain
Institut Pasteur [Paris]
Génomique Virale et Vaccination
Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
Immunité infection vaccination (I2V)
Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-Université de Lyon-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM)
Projets et développements en Bioinformatique
This work was supported in part by funding from the Institut Pasteur, and by grants from the Ligue Nationale Contre le Cancer (grants R05/75-129 and RS07/75-75), the Association pour la Recherche sur le Cancer (grants ARC A09/1/5031 and 4867), and the Agence Nationale de la Recherche (EPI-HPV-3D and NAB-HPV programs). G.N. was a recipient of the Conseil Général de la Vienne and Pasteur Weizmann foundation. C.R. was a recipient of a Ministère de l'Education Nationale de la Recherche et de la Technologie fellowship.
ANR-07-MIME-0009,EPI-HPV-3D,Vers la compréhension fonctionelle et structurale de l'interactome cellulaire des protéines précoces des Papilloma Virus Humains.(2007)
Institut Pasteur [Paris] (IP)
Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Source :
Nature Methods, Nature Methods, Nature Publishing Group, 2011, 8 (12), pp.2204-2207. ⟨10.1038/nmeth.1773⟩, Nature Methods, 2011, 8 (12), pp.2204-2207. ⟨10.1038/nmeth.1773⟩
Publication Year :
2011
Publisher :
Springer Science and Business Media LLC, 2011.

Abstract

International audience

Subjects

Subjects :
Proteomics
MESH: Copepoda
MESH: Databases, Protein
Luminescence
MESH: High-Throughput Screening Assays
Systems biology
Plasma protein binding
Computational biology
Biology
Biochemistry
Copepoda
03 medical and health sciences
MESH: Benchmarking
Protein-fragment complementation assay
Protein Interaction Mapping
High-Throughput Screening Assays
Animals
Humans
MESH: Protein Binding
MESH: Animals
Luciferase
Databases, Protein
Luciferases
Molecular Biology
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
MESH: Genetic Complementation Test
0303 health sciences
MESH: Humans
MESH: Proteomics
MESH: Luminescence
Genetic Complementation Test
MESH: Protein Interaction Mapping
030302 biochemistry & molecular biology
[SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN]
Cell Biology
Molecular biology
Benchmarking
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
MESH: Luciferases
Protein Binding
Biotechnology

Details

ISSN :
15487105 and 15487091
Volume :
8
Database :
OpenAIRE
Journal :
Nature Methods
Accession number :
edsair.doi.dedup.....3e0fa41f9a9714668a861e7dd7ecf1de
Full Text :
https://doi.org/10.1038/nmeth.1773
Full Text Access
View/download PDF

Tools

Authors Abstract Subjects Details