Back to Search
Start Over
A Cell-Based Self-Assembly Approach for the Production of Human Osseous Tissues from Adipose-Derived Stromal/Stem Cells
- Source :
- Advanced healthcare materials. 6(4)
- Publication Year :
- 2016
-
Abstract
- Achieving optimal bone defect repair is a clinical challenge driving intensive research in the field of bone tissue engineering. Many strategies focus on seeding graft materials with progenitor cells prior to in vivo implantation. Given the benefits of closely mimicking tissue structure and function with natural materials, the authors hypothesize that under specific culture conditions, human adipose-derived stem/stromal cells (hASCs) can solely be used to engineer human reconstructed osseous tissues (hROTs) by undergoing osteoblastic differentiation with concomitant extracellular matrix production and mineralization. Therefore, the authors are developing a self-assembly methodology allowing the production of such osseous tissues. Three-dimensional (3D) tissues reconstructed from osteogenically-induced cell sheets contain abundant collagen type I and are 2.7-fold less contractile compared to non-osteogenically induced tissues. In particular, hROT differentiation and mineralization is reflected by a greater amount of homogenously distributed alkaline phosphatase, as well as higher calcium-containing hydroxyapatite (P < 0.0001) and osteocalcin (P < 0.0001) levels compared to non-induced tissues. Taken together, these findings show that hASC-driven tissue engineering leads to hROTs that demonstrate structural and functional characteristics similar to native osseous tissue. These highly biomimetic human osseous tissues will advantageously serve as a platform for molecular studies as well as for future therapeutic in vivo translation.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
Stromal cell
Materials science
Cellular differentiation
Biomedical Engineering
Pharmaceutical Science
02 engineering and technology
Bone and Bones
Biomaterials
Extracellular matrix
03 medical and health sciences
Tissue engineering
medicine
Humans
Progenitor cell
Stem cell transplantation for articular cartilage repair
Osteoblasts
Tissue Engineering
Stem Cells
Mesenchymal stem cell
Cell Differentiation
021001 nanoscience & nanotechnology
Antigens, Differentiation
Cell biology
030104 developmental biology
Adipose Tissue
Female
Stem cell
0210 nano-technology
Subjects
Details
- ISSN :
- 21922659
- Volume :
- 6
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Advanced healthcare materials
- Accession number :
- edsair.doi.dedup.....3e2f5e4099ed7dd2d470f35675259fa6