Additional file 2: of Tumor-released autophagosomes induces CD4+ T cell-mediated immunosuppression via a TLR2–IL-6 cascade

Figure S1. Effect of TRAPs on differentiation of CD4+ T cells. Figure S2. Comparison of LC3B+ EVs and LC3B− EVs in instructing CD4+ T cells. Figure S3 TRAPs induce Akt/p38/NF-κB/STAT3 activation in CD4+ T cells via the TLR2–MyD88 pathway. Figure S4 IL-10 and IL-21 production by TRAPs-induced CD4+ T cells occurs via autocrine IL-6. Figure S5 Hsp90α on TRAPs is essential for the induction of IL-6 secretion from CD4+ T cells. Figure S6. The secretion of cytokines from TRAPs-induced CD4+ T cells (TTRAP) mediate IL-10-producing B cell differentiation. Figure S7 Evaluation of Beclin-1 expression in B16F10 cells. Figure S8 Depletion of selected cellular subsets during tumor growth. (DOCX 2.11 mb)