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Accumulation of a Brazil nut albumin in seeds of transgenic canola results in enhanced levels of seed protein methionine
- Source :
- Plant Molecular Biology. 18:235-245
- Publication Year :
- 1992
- Publisher :
- Springer Science and Business Media LLC, 1992.
-
Abstract
- We have increased the methionine content of the seed proteins of a commercial winter variety of canola by expressing a chimeric gene encoding a methionine-rich seed protein from Brazil nut in the seeds of transgenic plants. Transgenic canola seeds accumulate the heterologous methionine-rich protein at levels which range from 1.7% to 4.0% of the total seed protein and contain up to 33% more methionine. The precursor of the methionine-rich protein is processed correctly in the seeds, resulting in the appearance of the mature protein in the 2S protein fraction. The 2S methionine-rich protein accumulates in the transgenic seeds at the same time in development as the canola 11S seed proteins and disappears rapidly upon germination of the seed. The increase in methionine in the canola seed proteins should increase the value of canola meal which is used in animal feed formulations.
- Subjects :
- food.ingredient
Recombinant Fusion Proteins
Genetic Vectors
Immunoblotting
Colza oil
Gene Expression
Plant Science
Genetically modified crops
Biology
chemistry.chemical_compound
Methionine
Transformation, Genetic
food
Botany
Genetics
Nuts
Food science
Canola
Plant Proteins
Albumin
food and beverages
General Medicine
Plants, Genetically Modified
food.food
Transformation (genetics)
chemistry
Germination
Seeds
Agronomy and Crop Science
Plasmids
Brazil nut
Subjects
Details
- ISSN :
- 15735028 and 01674412
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Plant Molecular Biology
- Accession number :
- edsair.doi.dedup.....3e515f47a7c6709dbcdd9a3e5c21e93f