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Pathogenic Hydrogel? A Novel-Entrapment Neuropathy Model Induced by Ultrasound-Guided Perineural Injections
- Source :
- International Journal of Molecular Sciences, Volume 22, Issue 7, International Journal of Molecular Sciences, Vol 22, Iss 3494, p 3494 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Entrapment neuropathy (EN) is a prevalent and debilitative condition caused by a complex pathogenesis that involves a chronic compression–edema–ischemia cascade and perineural adhesion that results in excessive shear stress during motion. Despite decades of research, an easily accessible and surgery-free animal model mimicking the mixed etiology is currently lacking, thus limiting our understanding of the disease and the development of effective therapies. In this proof-of-concept study, we used ultrasound-guided perineural injection of a methoxy poly(ethylene glycol)-b-Poly(lactide-co-glycoilide) carboxylic acid (mPEG-PLGA-BOX) hydrogel near the rat’s sciatic nerve to induce EN, as confirmed sonographically, electrophysiologically, and histologically. The nerve that was injected with hydrogel appeared unevenly contoured and swollen proximally with slowed nerve conduction velocities across the injected segments, thus showing the compressive features of EN. Histology showed perineural cellular infiltration, deposition of irregular collagen fibers, and a possible early demyelination process, thus indicating the existence of adhesions. The novel method provides a surgery-free and cost-effective way to establish a small-animal model of EN that has mixed compression and adhesion features, thus facilitating the additional elucidation of the pathophysiology of EN and the search for promising treatments.
- Subjects :
- Male
Pathology
Compressive Strength
carpal tunnel syndrome
Adhesion (medicine)
Polyethylene Glycols
lcsh:Chemistry
Rats, Sprague-Dawley
Pathogenesis
0302 clinical medicine
Edema
lcsh:QH301-705.5
Myelin Sheath
Spectroscopy
030222 orthopedics
Chemistry
ultrasound
Ultrasound
Hydrogels
General Medicine
Sciatic Nerve
Pathophysiology
Computer Science Applications
Cellular infiltration
Ultrasonic Waves
Entrapment Neuropathy
Sciatic nerve
medicine.medical_specialty
Polyesters
entrapment neuropathy
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
medicine
Animals
Physical and Theoretical Chemistry
Molecular Biology
business.industry
Nerve Compression Syndromes
animal model
Organic Chemistry
Histology
medicine.disease
Rats
Disease Models, Animal
lcsh:Biology (General)
lcsh:QD1-999
Nervous System Diseases
hydrogel
business
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....3e647a2f2534c53aff094437e3acc8a6
- Full Text :
- https://doi.org/10.3390/ijms22073494