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Liquid Chromatography-Tandem Mass Spectrometry Simultaneous Determination and Pharmacokinetic Study of Fourteen Alkaloid Components in Dog Plasma after Oral Administration of Corydalis bungeana Turcz Extract
- Source :
- Molecules, Volume 23, Issue 8, Molecules, Vol 23, Iss 8, p 1927 (2018), Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
- Publication Year :
- 2018
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2018.
-
Abstract
- A rapid and sensitive Ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous determination of fourteen alkaloids in beagle dog plasma after a single oral dose of the Corydalis bungeana Turcz (C. bungeana) extract selected bifendate as the internal standard (IS). The plasma samples were preprocessed by liquid-liquid extraction (LLE) with aether before separation on an Agilent SB-C18 column (1.8 &micro<br />m, 150 &times<br />2.1 mm) using a gradient elution program. The mobile phase consists of 0.2% acetic acid and acetonitrile at the flow rate of 0.3 mL/min. In the positive ion mode, the analytes were detected by multiple reaction monitoring (MRM). The results indicated that calibration curves for fourteen analytes have good linearity (R2 = 0.9904). The lower limits of quantification (LLOQ) of fourteen alkaloids and IS were all over 4.87 ng/mL and the matrix effects ranged from 94.08% to 102.76%. The mean extraction recoveries of Quality control samples at low (LQC), medium (MQC) and high (HQC) and IS were all more than 78.03%. The intra- and inter-day precision (R.S.D.%) also met the criterion, at the same time the deviation of assay accuracies (R.E) ranged from &minus<br />13.70% to 14.40%. The Tmax values of fourteen alkaloids were no more than 1 h. The range of Cmax was from 74.16 &plusmn<br />8.71 to 2256 &plusmn<br />255.9 ng/mL. The assay was validated in the light of the regulatory bioanalytical guidelines and proved acceptable, which was successfully applied to a pharmacokinetic study of these compounds in beagle dogs after oral administration of Corydalis bungeana Turcz extract.
- Subjects :
- Male
0301 basic medicine
Bioanalysis
Cmax
Administration, Oral
Pharmaceutical Science
Mass spectrometry
alkaloids
Article
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
Dogs
0302 clinical medicine
lcsh:Organic chemistry
Pharmacokinetics
Limit of Detection
Tandem Mass Spectrometry
Liquid chromatography–mass spectrometry
Drug Discovery
Ultra high-performance liquid chromatography-tandem mass spectrometry
Animals
Physical and Theoretical Chemistry
Chromatography
Plant Extracts
Chemistry
Organic Chemistry
Selected reaction monitoring
Extraction (chemistry)
Reproducibility of Results
Reference Standards
030104 developmental biology
Corydalis
Corydalis bungeana Turcz
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
Calibration
Regression Analysis
Molecular Medicine
Quantitative analysis (chemistry)
pharmacokinetics
Chromatography, Liquid
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....3e7318f41a7ed35e820bee528d98355c
- Full Text :
- https://doi.org/10.3390/molecules23081927