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Cultured bone marrow cell local implantation accelerates healing of ulcers in mice
- Source :
- Journal of gastroenterology. 43(2)
- Publication Year :
- 2007
-
Abstract
- The therapeutic potential of bone marrow (BM)-derived cells in ulcers is not known. This study aimed to clarify (1) cell types that are derived from the BM which infiltrate ulcers; (2) whether BM-derived cells or gastric myofibroblasts can be used for cell transplantation to treat ulcers; and (3) the phenotypes of such transplantable cells.(1) Wild-type mice were transplanted with BM cells of green fluorescent protein (GFP)-transgenic mice. Acetic acid-induced gastric ulcers were produced in mice after BM transplantation. (2) BM cells and gastric myofibroblasts were isolated from GFP-transgenic mice. Bone marrow cells attached to plastic dishes were selected for expansion. Gastric ulcers were induced, and BM-derived cells, myofibroblasts, or phosphate-buffered saline were injected around ulcers. The ulcer healing process was examined macroscopically and histologically. (3) Expression of growth factors and cytokines in transplantable cells was examined by reverse transcriptase-polymerase chain reaction.(1) GFP-positive cells with interstitial phenotypes were observed at the ulcerated area. (2) Ulcer healing was significantly promoted by the injection of BM-derived cells compared to controls on day 7, but not on day 3. The BM-derived cells were observed in the tissue surrounding the ulcer. However, myofibroblasts were not found. (3) The BM-derived cells expressed hepatocyte growth factor, transforming growth factor-beta(1), and other stromal factors before transplantation, and had mesenchymal phenotypes after transplantation.BM-derived cells are involved in the ulcer healing. BM-derived cells, but not myofibroblasts, are locally implantable to ulcers. Thus, BM-derived cells can be transplanted to accelerate ulcer healing.
- Subjects :
- Male
Cell type
Pathology
medicine.medical_specialty
Stromal cell
Mice, Transgenic
Biology
Mice
medicine
Animals
Stomach Ulcer
Cells, Cultured
Acetic Acid
Bone Marrow Transplantation
Wound Healing
Mesenchymal stem cell
Transdifferentiation
Gastroenterology
digestive system diseases
Transplantation
medicine.anatomical_structure
Phenotype
Immunology
Cell Transdifferentiation
Hepatocyte growth factor
Female
Bone marrow
Stem cell
medicine.drug
Subjects
Details
- ISSN :
- 09441174
- Volume :
- 43
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of gastroenterology
- Accession number :
- edsair.doi.dedup.....3eaa9cdbf8e8e6907854d6b2b677c90b