Production and purification of antibody against protein-vitamin b12 conjugates for radioimmunoassay purposes

Antibodies have been evoked against vitamin B 12 conjugates using two types of antigen: intrinsic factor saturated with vitamin B 12 and vitamin B 12 covalently coupled to albumin. The antibodies were purified by rivanol and identified by polyacrylamide gel electrophoresis. Purified and unpurified anti-bodies were both applied in a radioimmunoassay for vitamin B 12 . Affinity chromatography proved to be a useful method for purification of the vitamin B 12 antibodies. An immunoadsorbent of vitamin B 12 covalently coupled to aminoethylcellulose was used.