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Production and purification of antibody against protein-vitamin b12 conjugates for radioimmunoassay purposes
- Source :
- Clinica chimica acta, 56(2), 143-149. ELSEVIER SCIENCE BV
- Publication Year :
- 1974
-
Abstract
- Antibodies have been evoked against vitamin B 12 conjugates using two types of antigen: intrinsic factor saturated with vitamin B 12 and vitamin B 12 covalently coupled to albumin. The antibodies were purified by rivanol and identified by polyacrylamide gel electrophoresis. Purified and unpurified anti-bodies were both applied in a radioimmunoassay for vitamin B 12 . Affinity chromatography proved to be a useful method for purification of the vitamin B 12 antibodies. An immunoadsorbent of vitamin B 12 covalently coupled to aminoethylcellulose was used.
- Subjects :
- Intrinsic Factor
Swine
Clinical Biochemistry
Radioimmunoassay
Diamines
Biochemistry
Antibodies
Chromatography, Affinity
Folic Acid
Antigen
Affinity chromatography
Ethylamines
Animals
Vitamin B12
Cobalt Radioisotopes
Cellulose
Polyacrylamide gel electrophoresis
Intrinsic factor
Chromatography
biology
Chemistry
Biochemistry (medical)
Albumin
Serum Albumin, Bovine
General Medicine
Electrophoresis, Disc
Vitamin B 12
Lactates
biology.protein
Acridines
Rabbits
Antibody
Protein Binding
Subjects
Details
- Language :
- English
- ISSN :
- 00098981
- Database :
- OpenAIRE
- Journal :
- Clinica chimica acta, 56(2), 143-149. ELSEVIER SCIENCE BV
- Accession number :
- edsair.doi.dedup.....3eab891a97b8c71c070433fed7f7cfd3