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Effectiveness of digital PCR for MYD88L265P detection in vitreous fluid for primary central nervous system lymphoma diagnosis
- Source :
- Experimental and Therapeutic Medicine
- Publication Year :
- 2020
- Publisher :
- D.A. Spandidos, 2020.
-
Abstract
- Primary central nervous system lymphoma (PCNSL) is a rare type of primary extranodal lymphoma (PEL). MYD88L265P mutation has been observed in up to 75% of PCNSL cases, however, the validity and sensitivity of digital PCR in detecting this mutation remains to be elucidated. A total of 44 PCNSL patients, 15 diffuse large B-cell lymphoma not otherwise specified (DLBCL-NOS) patients and 13 other PEL patients were enrolled in the present study. The abilities of reverse transcription quantitative PCR (RT-qPCR) and droplet digital PCR (ddPCR) to detect the MYD88L265P mutation in cerebral spinal fluid (CSF) samples were compared. The results suggested that ddPCR showed superior mutation detection sensitivity when compared with RT-qPCR (58 vs. 15%; P
- Subjects :
- 0301 basic medicine
Cancer Research
Pathology
medicine.medical_specialty
ddPCR
cerebrospinal fluid
03 medical and health sciences
0302 clinical medicine
Cerebrospinal fluid
Immunology and Microbiology (miscellaneous)
hemic and lymphatic diseases
medicine
Digital polymerase chain reaction
central nervous system lymphoma
business.industry
vitreous aspirates
Not Otherwise Specified
Primary central nervous system lymphoma
Cancer
General Medicine
Articles
medicine.disease
Lymphoma
030104 developmental biology
Real-time polymerase chain reaction
030220 oncology & carcinogenesis
MYD88L265P mutation
Mutation (genetic algorithm)
business
Subjects
Details
- Language :
- English
- ISSN :
- 17921015 and 17920981
- Volume :
- 20
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Experimental and Therapeutic Medicine
- Accession number :
- edsair.doi.dedup.....3ec4eca0e1903dcee8522980dab82991