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Construction of homo- and heteropolymers of plant ferritin subunits using an in vitro protein expression system
- Source :
- Protein expression and purification. 56(2)
- Publication Year :
- 2007
-
Abstract
- Ferritin is a class of iron storage protein composed of 24 subunits. Although many studies on gene expression analyses of plant ferritin have been conducted, the functions and oligomeric assembly of plant ferritin subunits are still largely unknown. In order to characterize the ability to form multimeric protein shells and determine the iron incorporating activity, we produced ferritin homo- and heteropolymers by expressing four cDNAs of ferritin subunits from soybean, sfer1, sfer2, sfer3, and sfer4, using an in vitro protein expression system. Using SDS–PAGE analysis followed by Prussian blue stain, homopolymers of SFER1, SFER2, and SFER3, and heteropolymers of SFER1/SFER2 and SFER1/SFER3 were detected as assembled polymers with iron incorporating activity, whereas only a small amount of SFER4 related homo- and heteropolymer was detected, suggesting that the SFER4 was not competent for oligomeric assembly, unlike every other ferritin. We conclude that certain combinations of plant ferritin subunits can form heteropolymers and that their iron incorporating activities depend on the formation of multimeric protein.
- Subjects :
- Multiprotein complex
DNA, Complementary
Iron
Genetic Vectors
Molecular Sequence Data
Biology
Protein Engineering
Iron storage
In vitro
Protein expression
Ferritin
Protein Subunits
Biopolymers
Biochemistry
Gene expression
Ferritins
biology.protein
Humans
Amino Acid Sequence
Perls' Prussian blue
Biotechnology
Plant Proteins
Subjects
Details
- ISSN :
- 10465928
- Volume :
- 56
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Protein expression and purification
- Accession number :
- edsair.doi.dedup.....3ededaf1afd081beb1fe4f57b7260d9c