Back to Search
Start Over
Intelligent demethylase-driven DNAzyme sensor for highly reliable metal-ion imaging in living cells
- Source :
- Chemical Science
- Publication Year :
- 2021
-
Abstract
- The accurate intracellular imaging of metal ions requires an exquisite site-specific activation of metal-ion sensors, for which the pervasive epigenetic regulation strategy can serve as an ideal alternative thanks to its orthogonal control feature and endogenous cell/tissue-specific expression pattern. Herein, a simple yet versatile demethylation strategy was proposed for on-site repairing-to-activating the metal-ion-targeting DNAzyme and for achieving the accurate site-specific imaging of metal ions in live cells. This endogenous epigenetic demethylation-regulating DNAzyme system was prepared by modifying the DNAzyme with an m6A methylation group that incapacitates the DNAzyme probe, thus eliminating possible off-site signal leakage, while the cell-specific demethylase-mediated removal of methylation modification could efficiently restore the initial catalytic DNAzyme for sensing metal ions, thus allowing a high-contrast bioimaging in live cells. This epigenetic repair-to-activate DNAzyme strategy may facilitate the robust visualization of disease-specific biomarkers for in-depth exploration of their biological functions.<br />A simple yet versatile demethylation strategy is proposed for an on-site repairing-to-activating metal-ion-targeting DNAzyme and for achieving the highly reliable site-specific imaging of metal ions in live cells.
Details
- ISSN :
- 20416520
- Volume :
- 12
- Issue :
- 46
- Database :
- OpenAIRE
- Journal :
- Chemical science
- Accession number :
- edsair.doi.dedup.....3f0af97b1690973cfcf7c05faaa36554