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Genome and transcriptome analysis of surfactin biosynthesis in Bacillus amyloliquefaciens MT45
- Source :
- Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Natural Bacillus isolates generate limited amounts of surfactin (Bacillus amyloliquefaciens MT45 was observed at a titre of 2.93 g/l, which is equivalent to half of the maximum biomass. To systemically unravel this efficient biosynthetic process, the genome and transcriptome of this bacterium were compared with those of B. amyloliquefaciens type strain DSM7T. MT45 possesses a smaller genome while containing more unique transporters and resistance-associated genes. Comparative transcriptome analysis revealed notable enrichment of the surfactin synthesis pathway in MT45, including central carbon metabolism and fatty acid biosynthesis to provide sufficient quantities of building precursors. Most importantly, the modular surfactin synthase overexpressed (9 to 49-fold) in MT45 compared to DSM7T suggested efficient surfactin assembly and resulted in the overproduction of surfactin. Furthermore, based on the expression trends observed in the transcriptome, there are multiple potential regulatory genes mediating the expression of surfactin synthase. Thus, the results of the present study provide new insights regarding the synthesis and regulation of surfactin in high-producing strain and enrich the genomic and transcriptomic resources available for B. amyloliquefaciens.
- Subjects :
- 0301 basic medicine
Bacillus amyloliquefaciens
030106 microbiology
Peptides, Cyclic
Genome
Article
Transcriptome
Lipopeptides
03 medical and health sciences
chemistry.chemical_compound
Gene Regulatory Networks
Overproduction
Gene
Regulator gene
Multidisciplinary
biology
Gene Expression Profiling
Genomics
biology.organism_classification
030104 developmental biology
chemistry
Biochemistry
Biosynthetic process
lipids (amino acids, peptides, and proteins)
Surfactin
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....3f3414688138a04641e1ff4f0db73af9